An antigenic peptide inducing cross-reacting antibodies inhibiting the interaction of Streptococcus mutans PAc with human salivary components
An antigenic peptide inducing cross-reacting antibodies inhibiting the interaction of Streptococcus mutans PAc with human salivary components
复制标题
一种抗原肽诱导交叉反应抗体，抑制变形链球菌 PAc 与人唾液成分的相互作用
作者：
H. Senpuku;Takako Miyauchi;Nobuhiro Hanada;T. Nisizawa
变形链球菌的190-kDa表面蛋白抗原（PAc），特别是该分子的A区，可能通过与唾液组分的相互作用参与龋齿的诱导。由于这个原因，它可能被成功地用作实验性疫苗接种的抗原成分，以预防动物的龋齿。在开发用于龋齿的合成肽疫苗时，如本文所报道的，我们已经鉴定了独特的肽TYEAALKQYEADL作为候选疫苗免疫原。该肽的氨基酸序列完全对应于PAc的A区中的B细胞表位的序列，并且在分子内另外含有其自身的B10.D2小鼠的T细胞表位。该肽强烈诱导仅针对PAc的交叉反应抗体的产生。此外，如通过使用BIAcore系统的表面等离子体共振分析所证明的，这些交叉反应抗体抑制约50%的液相唾液组分与固定化重组PAc的结合。
A 190-kDa surface protein antigen (PAc) of Streptococcus mutans, in particular the A region of this molecule, may be implicated in the induction of dental caries via an interaction with salivary components. For this reason, it was probably used successfully as an antigenic component for experimental vaccination to prevent dental caries in animals. While developing a synthetic peptide vaccine for dental caries, as reported herein, we have identified a unique peptide, TYEAALKQYEADL, as a candidate vaccinal immunogen. The amino acid sequence of this peptide completely corresponds to the sequence of a B-cell epitope in the A region of PAc and additionally contains its own T-cell epitope for B10.D2 mice within the molecule. This peptide strongly induces the production of only cross-reacting antibodies against PAc. In addition, as demonstrated by surface plasmon resonance analysis using the BIAcore system, these cross-reacting antibodies inhibit approximately 50% of the binding of fluid-phase salivary components to immobilized recombinant PAc.
影响因子：
4.4
作者：
B. Nardelli;Y. A. Lu;D. R. Shiu;C. Delpierre-Defoort;A. Profy;J. Tam
通讯作者：
B. Nardelli;Y. A. Lu;D. R. Shiu;C. Delpierre-Defoort;A. Profy;J. Tam
干扰天然加工的肽与 II 类的结合会改变体内 T 细胞表位的免疫优势。
DOI：
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发表时间：
1994
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Nikcevich,KM;Kopielski,D;Finnegan,A
通讯作者：
Finnegan,A
链球菌表面蛋白抗原的合成肽的免疫原性和对变形链球菌口腔定植的保护作用。
DOI：
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发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Takahashi,I;Okahashi,N;Matsushita,K;Tokuda,M;Kanamoto,T;Munekata,E;Russell,MW;Koga,T
通讯作者：
Koga,T