Emergence of a retrovirus in a cloned cell line established from a lesion of Hodgkin's disease.
Emergence of a retrovirus in a cloned cell line established from a lesion of Hodgkin's disease.
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从霍奇金病病变建立的克隆细胞系中出现逆转录病毒。
DOI：
10.1002/hon.2900060304
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发表时间：
1988
影响因子：
3.3
通讯作者：
Behnke,O
中科院分区：
文献类型：
--
作者：
Olsson,L;Behnke,O
人逆转录病毒的存在仍然难以捉摸，并且经常是有争议的，直到1979年，当第一个人逆转录病毒HTLV-I被鉴定时（Poiesz等人，1981年，1982年）。HTLV-I的鉴定作为鉴定其它逆转录病毒如HTLV-I1和HIV的动力（Gallo et al.，1984年; Wong-Staal和Gallo，1985年）。鉴定HTLV-I所需的实验条件说明了在以前的实验中可能阻碍成功证明逆转录病毒在任何人类癌症中的许多因素。因此，在20世纪70年代中期，人们经常认为人类逆转录病毒不存在，因为如果它们存在的话，它们已经被鉴定出来了，而且因为在任何癌症患者中都没有检测到针对逆转录病毒成分的可检测抗体反应。回想起来，人们可能会问，为什么所有旨在识别人类逆转录病毒的实验都失败了。如果人类白血病细胞不能通过加入白细胞介素-2作为生长因子繁殖许多代，那么HTLV-I就很难被鉴定出来，因为逆转录病毒只有在体外多次传代后才出现（4和60）。这些病毒以前已经出现在许多培养物中，并且最常被鉴定为鼠污染物。1982年）。多年来，曾报道过霍奇金病的聚集性病例（Clemmesen，1981），但试图证明与该疾病相关的逆转录病毒均失败（Kaplan，1980）。一个主要障碍是难以从霍奇金病细胞建立永久性细胞系。直到最近才描述了几种细胞系，包括来自我们自己实验室的一种（Olsson，1985; Olsson等人，1984; Olsson和Behnke，1985），并且关于在这样的细胞中存在逆转录病毒感染的实验成为可能。我们在其他地方已经报道过小学的考试。关于逆转录病毒活性的霍奇金病组织培养物，发现所有这些研究都是阴性的（Kaplan等，1982年）。我们现在描述的出现逆转录病毒的起源不明克隆细胞系从脾病变结节硬化霍奇金病。的
The existence of human retroviruses remained elusive, and often debatable, until 1979, when the first human retrovirus, HTLV-I was identified (Poiesz et al., 1981, 1982). The identification of HTLV-I served as an impetus for the identification of other retroviruses, such as HTLV-I1 and HIV (Gallo et al., 1984; Wong-Staal and Gallo, 1985) that rapidly followed. The experimental conditions required for the identification of HTLV-I illustrated the many factors that in previous experiments may have hampered success in demonstrating retroviruses in any human cancer. Thus, in the mid 1970s it was often argued that human retroviruses did not exist because they would already have been identified if they existed, and because no detectable antibody response against retrovirus components could be detected in any cancer patients. In retrospect, one may ask why all the experiments aiming at identifying human retroviruses failed. HTLV-I would hardly have been identified, if human leukemic cells could not have been propagated in many generations by the addition of interleukin-2 as growth factor, because a retrovirus emerged only after numerous in vitro passages (4&60). Such viruses had emerged in many cultures previously and had most often been identified as murine contaminants.Hodgkin’s disease has for many years been thought to involve a retrovirus in its oncogenesis (Kaplan et al., 1982). Cluster cases of Hodgkin’s disease have been reported (Clemmesen, 1981) over the years, but attempts to demonstrate a retrovirus related to this disease have all failed (Kaplan, 1980). One main obstacle has been difficulties in establishing permanent cell lines from Hodgkin’s disease cells. Only recently have several cell lines been described, including one from our own laboratory (Olsson, 1985; Olsson et al., 1984; Olsson and Behnke, 1985), and experimentation concerning the presence of retrovirus infection in such cells became possible. We have reported elsewhere on the examination of primary. cultures of Hodgkin’s disease tissues in regard to retrovirus activity and found that all such investigations were negative (Kaplan et al., 1982). We now describe the emergence of a retrovirus of unknown origin in a cloned cell line from a splenic lesion of nodular sclerosis Hodgkin’s disease. The