Over-expression of lysophosphatidic acid receptor-2 in human invasive ductal carcinoma.
Over-expression of lysophosphatidic acid receptor-2 in human invasive ductal carcinoma.
复制标题
人浸润性导管癌中溶物磷脂酸受体-2的过表达。
DOI：
10.1186/bcr935
复制
发表时间：
2004
影响因子：
7.4
通讯作者：
Nagawa, H
中科院分区：
文献类型：
--
作者：
Kitayama, J;Shida, D;Sako, A;Ishikawa, M;Hama, K;Aoki, J;Arai, H;Nagawa, H
溶血磷脂酸（LPA）是一种生物活性磷脂，对多种细胞具有不同的作用。它与至少三种G蛋白偶联跨膜受体，即LPA 1，LPA 2和LPA 3相互作用，其在各种肿瘤中的表达尚未完全表征。在本研究中，我们表征了LPA受体在人乳腺癌组织中的表达谱，并评估了每个受体的可能作用。采用实时荧光定量RT-PCR技术检测了手术切除的浸润性导管癌和正常腺体组织中各受体mRNA相对于β-actin mRNA的表达水平。还使用大鼠抗LPA 2单克隆抗体以化学方法检测LPA 2表达。25例癌组织和正常组织中LPA 1 mRNA的表达水平相近，而癌组织中LPA 2 mRNA的表达水平明显高于正常组织（3479.0 ± 426.6比1287.3 ± 466.8; P < 0.05）。LPA 3在癌组织和正常腺体组织中均呈弱表达。免疫组化结果显示，在84例乳腺癌中，48例（57%）的乳腺癌细胞中LPA 2蛋白的表达较正常乳腺上皮增强。38例绝经前妇女中17例（45%）LPA 2过表达，46例绝经后妇女中31例（67%）LPA 2过表达，差异有统计学意义（P < 0.05）。这些发现表明，LPA 2的上调可能在致癌作用中发挥作用，特别是在绝经后乳腺癌中。
Lysophosphatidic acid (LPA) is a bioactive phospholipid with diverse effects on various cells. It interacts with at least three G-protein-coupled transmembrane receptors, namely LPA1, LPA2 and LPA3, whose expression in various tumours has not been fully characterized. In the present study we characterized the expression profile of LPA receptors in human breast cancer tissue and assessed the possible roles of each receptor. The relative expression levels of each receptor's mRNA against β-actin mRNA was examined in surgically resected invasive ductal carcinomas and normal gland tissue using real-time RT-PCR. LPA2 expression was also examined immunohistochemically using a rat anti-LPA2 monoclonal antibody. In 25 cases normal and cancer tissue contained LPA1 mRNA at similar levels, whereas the expression level of LPA2 mRNA was significantly increased in cancer tissue as compared with its normal counterpart (3479.0 ± 426.6 versus 1287.3 ± 466.8; P < 0.05). LPA3 was weakly expressed in both cancer and normal gland tissue. In 48 (57%) out of 84 cases, enhanced expression of LPA2 protein was confirmed in carcinoma cells as compared with normal mammary epithelium by immunohistochemistry. Over-expression of LPA2 was detected in 17 (45%) out of 38 premenopausal women, as compared with 31 (67%) out of 46 postmenopausal women, and the difference was statistically significant (P < 0.05). These findings suggest that upregulation of LPA2 may play a role in carcinogenesis, particularly in postmenopausal breast cancer.
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DOI：
10.1073/pnas.97.24.13384
发表时间：
2000-11-21
影响因子：
11.1
作者：
Contos, JJA;Fukushima, N;Chun, J
通讯作者：
Chun, J
影响因子：
120.7
作者：
Holmes, MD;Hunter, DJ;Hankinson, SE
通讯作者：
Hankinson, SE
影响因子：
158.5
作者：
Hunter, DJ;Spiegelman, D;Willett, W
通讯作者：
Willett, W
影响因子：
4.8
作者：
Bandoh, K;Aoki, J;Inoue, K
通讯作者：
Inoue, K
影响因子：
29.4
作者：
Deng, WL;Balazs, L;Johnson, LR
通讯作者：
Johnson, LR