Differential expression of growth factors at the cellular level in virus-infected brain.
Differential expression of growth factors at the cellular level in virus-infected brain.
复制标题
病毒感染的大脑中生长因子在细胞水平上的差异表达。
DOI：
10.1073/pnas.0430999100
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发表时间：
2003
影响因子：
11.1
通讯作者：
Hooper,DCraig
中科院分区：
文献类型：
--
作者：
Prosniak,Mikhail;Zborek,Anna;Scott,GwenS;Roy,Anirban;Phares,TimothyW;Koprowski,Hilary;Hooper,DCraig
宿主因子对狂犬病毒（RV）转录/复制和轴突/跨突触传播的贡献在很大程度上是未知的。 我们以前确定了几个宿主基因在RV感染期间在小鼠大脑中上调，包括神经白细胞介素，它参与神经元的生长和存活，细胞运动和分化，成纤维细胞生长因子同源因子4（FHF 4），这与肢体和神经系统发育有关。在这项研究中，我们使用实时定量RT-PCR来评估神经白细胞介素，FHF 4基因编码的FHF 4的两种亚型（FHF 4 -1a和-1b），和RV的N蛋白在神经元和星形胶质细胞中的表达，这些神经元和星形胶质细胞是通过激光捕获显微切割从实验室适应的RV毒株CVS-N2 c或银发蝙蝠来源的街头RV感染的小鼠大脑中分离出来的。基因表达模式的差异表明，RV毒株感染非神经元细胞和差异调节宿主基因表达的能力可能是重要的病毒复制和传播的中枢神经系统。
The contribution of host factors to rabies virus (RV) transcription/replication and axonal/transsynaptic spread is largely unknown. We previously identified several host genes that are up-regulated in the mouse brain during RV infection, including neuroleukin, which is involved in neuronal growth and survival, cell motility, and differentiation, and fibroblast growth factor homologous factor 4 (FHF4), which has been implicated in limb and nervous system development. In this study, we used real-time quantitative RT-PCR to assess the expression of mRNAs specific for neuroleukin, the two isoforms of FHF4 (FHF4-1a and -1b) encoded by the FHF4 gene, and N protein of RV in neurons and astrocytes isolated by laser capture microdissection from mouse brains infected with the laboratory-adapted RV strain CVS-N2c or with a street RV of silver-haired bat origin. Differences in the gene expression patterns suggest that the capacity of RV strains to infect nonneuronal cells and differentially modulate host gene expression may be important in virus replication and spread in the CNS.