Limited detection of sternal bone marrow infectivity in the clinical phase of experimental bovine spongiform encephalopathy (BSE)
Limited detection of sternal bone marrow infectivity in the clinical phase of experimental bovine spongiform encephalopathy (BSE)
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实验性牛海绵状脑病（BSE）临床阶段胸骨骨髓感染性检测有限
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
M. Dawson
中科院分区：
文献类型：
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作者：
G. Wells;S. Hawkins;R. Green;Y. Spencer;I. Dexter;M. Dawson
最近发表的一份报告(Wells和其他1998)更新了一项连续时间点研究的中期结果，该研究正在研究口服感染牛海绵状脑病(BSE)病原体的牛的传染性传播和病理变化的发展(Wells等人1994,1996)。这些先前的结果描述了牛的检查时间表，牛在口服接触后2至40个月被杀，以及牛在接触后35至37个月的临床体征的发展。通过小鼠生物测定，它们还在以下部位显示了感染性：回肠远端(暴露后6~18个月、38个月和40个月采集)；中枢神经系统脑和脊髓(暴露后32~40个月采集)；感觉神经节背根神经节(暴露后32~40个月采集)和三叉神经节(暴露后36个月和38个月采集)。在截至1997年6月完成化验的35个剩余组织中(即在接触病毒后2至22个月从牛身上抽取的组织)中，没有发现任何组织具有传染性。对研究中所有连续杀死时间点的大范围组织的小鼠生物分析现已完成(1998年12月)，并将在其他地方完整报告。这篇简短的通讯报告了更多关于C57B1-J6小鼠骨髓生物测定的数据，完成了研究中所有牛的这一组织的结果(Wells和其他1998年)。这项研究的实验设计的细节已经在前面描述过了(Wells等人1996、1998)。从胸骨(来自第三或第四胸骨中心的松质骨)采集骨髓，作为每个组织的样本。
A RECENTLY published report (Wells and others 1998) updated interim findings in a sequential time point study which is examining the spread of infectivity and development of pathological changes in cattle exposed orally to infection with the agent of bovine spongiform encephalopathy (BSE) (Wells and others 1994, 1996). These previous results described the schedule of examination of cattle, killed from two to 40 months after oral exposure, and the development of clinical signs in cattle 35 to 37 months after the exposure. They also demonstrated infectivity by mouse bioassay in: distal ileum (sampled from cattle six to 18 months, 38 months and 40 months after exposure); central nervous system brain and spinal cord (sampled from cattle 32 to 40 months after exposure); and sensory ganglia dorsal root ganglia (sampled from cattle 32 to 40 months after exposure) and trigeminal ganglion (sampled from cattle 36 months and 38 months after exposure). No infectivity had been detected in any of the 35 remaining tissues for which assays were complete at June 1997 (that is, those sampled from cattle two to 22 months after exposure). Mouse bioassays of a large range of tissues from all sequential kill time points of the study have now been completed (at December 1998) and will be reported in full elsewhere. This short communication reports additional data on the bioassay in C57B1-J6 mice of bone marrow, completing results for this tissue from all cattle in the study (Wells and others 1998). Details of the experimental design of the study have been described previously (Wells and others 1996,1998). Bone marrow from the sternum (cancellous bone from the centre of the third or fourth sternebra) was sampled, as for each of the tis-