Comparative study of cytoplasmic elimination in spermatids of selected mammalian species.
Comparative study of cytoplasmic elimination in spermatids of selected mammalian species.
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DOI：
10.1002/aja.1001780109
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Robert L. Sprando;Lonnie D. Russell;Lonnie D. Russell
中科院分区：
文献类型：
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作者：
Robert L. Sprando;Lonnie D. Russell;Lonnie D. Russell
在精子细胞转变为精子的过程中，会发生细胞质损失，从而使精子能够不受阻碍地游动。细胞质损失是通过消除残留体并通过称为管球复合体的结构而发生的。使用高分辨率电子显微镜形态测量技术测定了几个物种的细胞质损失。在管球复合体存在期间，五个物种的细胞质损失平均为 53%（豚鼠，48.8 +/- 6.2% (SEM)；猴，60.3 +/- 4.3%；负鼠，54.5 +/- 4.4%；兔，46.9 +/- 2.7%；大鼠，55.7 +/- 4.9%），而在同一时期，圆形精子细胞的细胞质基本上没有损失或增加。令人惊讶的是，在精细胞伸长过程中，五个物种也记录了大约 36% 的细胞质损失（豚鼠，50.1 +/- 6.3%；猴，30.0 +/- 15.4%；负鼠，25.4 +/- 9.0%；兔，42.4 +/- 8.6%；大鼠，34.9 +/- 11.9%），这是仅部分（约 60%）是由核凝聚过程中从核中泵出的流体造成的。与圆形精子细胞相比，细长精子细胞的细胞质致密化表明液体也从细长精子细胞细胞质中泵出。生殖细胞的液体流失可能会导致生精小管液体的产生，这种液体以前被认为仅来源于支持细胞。
In the transformation of a spermatid into a spermatozoon, cytoplasmic loss occurs, allowing the sperm to swim unhindered. Cytoplasmic loss takes place via elimination of the residual body and through structures known as tubulobulbar complexes. A determination of cytoplasmic loss in several species was undertaken by using high-resolution electron microscopic morphometric techniques. During the period that tubulobulbar complexes are present, an average 53% cytoplasmic loss was recorded for five species (guinea pig, 48.8 +/- 6.2% (SEM); monkey, 60.3 +/- 4.3%; opossum, 54.5 +/- 4.4%; rabbit, 46.9 +/- 2.7%; and rat, 55.7 +/- 4.9%), whereas there was essentially no loss or gain in cytoplasm during the same period for round spermatids. Surprisingly, during spermatid elongation an approximate 36% loss of cytoplasm was also recorded for five species (guinea pig, 50.1 +/- 6.3%; monkey, 30.0 +/- 15.4%; opossum, 25.4 +/- 9.0%; rabbit, 42.4 +/- 8.6%; and rat, 34.9 +/- 11.9%), which is only partially (approximately 60%) accounted for by fluid pumping from the nucleus during nuclear condensation. A densification of the cytoplasm of elongate spermatids, as compared with round spermatids, suggests fluid is also pumped from the elongating spermatid cytoplasm. Fluid loss from germ cells may contribute to the seminiferous tubule fluid, a fluid previously thought to be solely of Sertoli-cell origin.