Migration of germline progenitor cells is directed by sphingosine-1-phosphate signalling in a basal chordate.
Migration of germline progenitor cells is directed by sphingosine-1-phosphate signalling in a basal chordate.
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种系祖细胞的迁移是通过基底脊椎动物中的鞘氨醇1-磷酸信号传导指导的。
DOI：
10.1038/ncomms9565
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发表时间：
2015-10-12
影响因子：
16.6
通讯作者：
De Tomaso AW
中科院分区：
文献类型：
--
作者：
Kassmer SH;Rodriguez D;Langenbacher AD;Bui C;De Tomaso AW
殖民地海鞘Botryllus schlosseri在无性出芽过程中不断再生整个身体。新发育体的生殖细胞系来源于迁移的生殖细胞前体，但控制这一归巢过程的信号尚不清楚。在这里，我们表明，生殖细胞前体可以前瞻性地分离基于醛脱氢酶和整合素α-6的表达，这些细胞表达生殖细胞标志物，如vasa，pumilio和piwi，以及鞘氨醇-1-磷酸受体。在体外，鞘氨醇-1-磷酸（S1 P）刺激生殖细胞的迁移，这取决于整合素α-6活性。在体内，S1 P信号传导对于生殖细胞归巢到新发育的身体是必需的。S1 P由发育中的生殖细胞龛中的鞘氨醇激酶产生，并由体细胞组织中的脂质磷酸磷酸酶降解。这些结果表明，以前未知的作用，S1 P信号通路在生殖细胞迁移的海鞘Botryllus schlosseri。 生殖细胞迁移的调控在殖民海鞘Botryllusschlosseri知之甚少。在这个脊索动物中，Kassmer等人鉴定了鞘氨醇-1-磷酸在体外调节生殖细胞迁移和活生物体中细胞向新发育体的归巢。
The colonial ascidian Botryllus schlosseri continuously regenerates entire bodies in an asexual budding process. The germ line of the newly developing bodies is derived from migrating germ cell precursors, but the signals governing this homing process are unknown. Here we show that germ cell precursors can be prospectively isolated based on expression of aldehyde dehydrogenase and integrin alpha-6, and that these cells express germ cell markers such as vasa, pumilio and piwi, as well as sphingosine-1-phosphate receptor. In vitro, sphingosine-1-phosphate (S1P) stimulates migration of germ cells, which depends on integrin alpha-6 activity. In vivo, S1P signalling is essential for homing of germ cells to newly developing bodies. S1P is generated by sphingosine kinase in the developing germ cell niche and degraded by lipid phosphate phosphatase in somatic tissues. These results demonstrate a previously unknown role of the S1P signalling pathway in germ cell migration in the ascidian Botryllus schlosseri. The regulation of germ cell migration in the colonial ascidian Botryllus schlosseri is poorly understood. In this chordate, Kassmer et al. identify sphingosine-1-phosphate as regulating germ cell migration in vitro and homing of cells to newly developing bodies in live organisms.