Identification and expression of a new Ly6 gene cluster in zebrafish Danio rerio, with implications of being involved in embryonic immunity
Identification and expression of a new Ly6 gene cluster in zebrafish Danio rerio, with implications of being involved in embryonic immunity
复制标题
斑马鱼斑马鱼中新 Ly6 基因簇的鉴定和表达及其参与胚胎免疫的意义
DOI：
10.1016/j.fsi.2016.04.005
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发表时间：
2016
影响因子：
4.7
通讯作者：
Li Hongyan
中科院分区：
文献类型：
--
作者：
Wang Man;Li Lingyi;Guo Quanyang;Zhang Shicui;Ji Dongrui;Li Hongyan
淋巴细胞抗原-6（Ly 6）超家族是一个蛋白质大家族，其特征在于精确间隔的半胱氨酸基序，称为三指折叠。迄今为止，在许多物种中鉴定了大量Ly 6/uPAR家族成员。在这项研究中，我们首次报告的鉴定和表征的分泌Ly2.1-3蛋白的2号染色体上的斑马鱼，并确定表达模式。Ly2.1-3均具有保守的LU结构域，并采用与人CD 59、SLURP 1和其他Ly 6家族成员相似的三指结构。Ly2.1-3聚簇在2号染色体上，同源性较高，可能来源于染色体基因重复。Ly2.1-3在内胚层中表现出不同的表达模式，它们分别在消化道、肝脏和胰腺中大量且特异地表达。这种差异表达模式可能表明Ly2.1-3在基因复制过程中获得了新的功能。Ly2.1-3在LPS刺激后表达上调，提示Ly2.1-3在内毒素刺激的早期消化系统天然免疫应答中发挥作用。
Lymphocyte antigen-6 (Ly6) superfamily is a large family of proteins and characterized by precisely spaced cysteine motifs, termed the three-finger fold. To date, a large number of members of the Ly6/uPAR family were identified among many species. In this study, we first report the identification and characterization of the secreted Ly2.1-3 proteins on the chromosome 2 in zebrafish and determine the expression pattern. Ly2.1-3 all possess a conserved LU domain and adopt similar three-finger structure with human CD59, SLURP1 and other Ly6 family members. Ly2.1-3 cluster on chromosome 2 and share high homology, possibly originated from chromosomal gene duplication. Ly2.1-3 exhibit distinct expression pattern in the endoderm, they were found abundantly and specifically in the digestive tract, liver and pancreas respectively. The differential expression pattern may suggest Ly2.1-3 acquire new function during gene duplication. The expression level of Ly2.1-3 were up-regulating challenged with LPS indicated that they have a role in innate immune responses of the digestive system during endotoxin challenge in early stage.