RNA-sequencing elucidates the regulation of behavioural transitions associated with the mating process in honey bee queens.
RNA-sequencing elucidates the regulation of behavioural transitions associated with the mating process in honey bee queens.
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DOI：
10.1186/s12864-015-1750-7
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发表时间：
2015-07-31
期刊：
影响因子：
4.4
通讯作者：
Oldroyd BP
中科院分区：
文献类型：
--
作者：
Manfredini F;Brown MJ;Vergoz V;Oldroyd BP
交配是一个复杂的过程，经常与行为和生理变化有关。然而，对这些变化的遗传基础的理解是有限的。蜜蜂既是行为基因组学的模型系统，也是人类作物的主要授粉者;因此，了解交配过程既有纯粹的价值，也有应用价值。我们使用下一代转录组学来探测蜜蜂蜂王大脑中基因表达的变化，因为它们从处女过渡到交配繁殖状态。此外，我们使用CO2麻醉，诱导产卵没有交配，隔离的生殖成熟的过程。交配过程中产生的视觉，化学感受，代谢和免疫相关基因的表达显着变化。这些基因的差异表达清楚地映射到已知的行为和生理变化，这些变化发生在从处女女王到新交配女王的转变过程中。这些基因表达变化的一个子集也检测到在CO2处理的皇后，从以前的生理研究预测。此外，我们将我们的结果与以前使用微阵列技术在一系列实验时间点的研究进行了比较。免疫和视觉相关基因表达的变化在所有研究中都是共同的，支持这些基因组参与交配过程。我们的研究是理解自然系统中调节交配后行为转变的分子机制的重要一步。基因表达模式与先前研究的弱重叠表明了全基因组方法的高灵敏度。因此，虽然我们建立在以前的微阵列研究，探索蜜蜂交配后的变化，更广泛的实验设计，使用RNA测序，并专注于澳大利亚蜜蜂，这仍然没有破坏性的寄生虫瓦螨destructor，在目前的研究中，提供了独特的见解蜜蜂交配过程的生物学。本文的在线版本（doi：10.1186/s12864-015-1750-7）包含补充材料，可供授权用户使用。
Mating is a complex process, which is frequently associated with behavioural and physiological changes. However, understanding of the genetic underpinnings of these changes is limited. Honey bees are both a model system in behavioural genomics, and the dominant managed pollinator of human crops; consequently understanding the mating process has both pure and applied value. We used next-generation transcriptomics to probe changes in gene expression in the brains of honey bee queens, as they transition from virgin to mated reproductive status. In addition, we used CO2-narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation. The mating process produced significant changes in the expression of vision, chemo-reception, metabolic, and immune-related genes. Differential expression of these genes maps clearly onto known behavioural and physiological changes that occur during the transition from being a virgin queen to a newly-mated queen. A subset of these changes in gene expression were also detected in CO2-treated queens, as predicted from previous physiological studies. In addition, we compared our results to previous studies that used microarray techniques across a range of experimental time-points. Changes in expression of immune- and vision-related genes were common to all studies, supporting an involvement of these groups of genes in the mating process. Our study is an important step in understanding the molecular mechanisms regulating post-mating behavioural transitions in a natural system. The weak overlap in patterns of gene expression with previous studies demonstrates the high sensitivity of genome-wide approaches. Thus, while we build on previous microarray studies that explored post-mating changes in honey bees, the broader experimental design, use of RNA-sequencing, and focus on Australian honey bees, which remain free from the devastating parasite Varroa destructor, in the current study, provide unique insights into the biology of the mating process in honey bees. The online version of this article (doi:10.1186/s12864-015-1750-7) contains supplementary material, which is available to authorized users.