The effect of vascular endothelial growth factor on a rat model of traumatic arteriogenic erectile dysfunction
The effect of vascular endothelial growth factor on a rat model of traumatic arteriogenic erectile dysfunction
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DOI：
10.1016/s0022-5347(01)69141-9
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发表时间：
2002-02-01
影响因子：
6.6
通讯作者：
Lue, TF
中科院分区：
文献类型：
--
作者：
Lee, MC;El-Sakka, AI;Lue, TF
目的：我们测试了海绵体内注射血管内皮生长因子 (VEGF) 可以在创伤性动脉性勃起功能障碍大鼠模型中恢复勃起功能的假设。材料和方法：对 50 只 3 个月大的雄性大鼠进行双侧髂内动脉探查。共44只大鼠进行双侧髂内动脉结扎，6只进行探查作为假手术组。几分钟后，向16只大鼠海绵体内注射磷酸盐缓冲盐水(PBS)加牛血清白蛋白，每次2杯。 12 杯和 4 杯中含有 VEGF 加 PBS 加 BSA。进行 16 次 VEGF 加 PBS 加 BSA 检测。在第1、2和6周时，每组中约三分之一的大鼠接受海绵体神经电刺激以评估勃起功能，然后处死。收集阴茎组织进行组织化学和电子显微镜检查。结果：在假手术大鼠中未发现勃起功能受损。动脉结扎后，所有大鼠立即对神经刺激表现出很少或没有勃起反应。在 PBS 处理的大鼠中，在第 6 周注意到勃起功能的适度恢复。在 VEGF 处理的大鼠中，在第 1 周和第 2 周的第 4 杯中注意到勃起功能的显着恢复。仅在第 6 周时在第 2 和第 4 杯中进行分组。组。神经元一氧化氮合酶染色显示，第 1 周时，背侧或海绵体内神经中的神经元一氧化氮合酶阳性神经纤维减少。在 2 公斤和 4 公斤时，注意到神经元一氧化氮合酶阳性神经纤维的中度恢复。 VEGF 治疗组但 PBS 治疗组没有。电镜观察显示假手术大鼠没有病理变化。在结扎加 PBS 处理的大鼠中，在背神经中观察到有髓和无髓神经纤维萎缩。在 VEGF 治疗的大鼠中观察到部分恢复。在 PBS 中观察到分散的萎缩性平滑肌细胞，偶尔在 VEGF 治疗的大鼠中观察到，但在假手术组中未观察到。 VEGF 治疗大鼠中最引人注目的发现是内皮细胞肥大和增生，尤其是小毛细血管内皮细胞。 结论：结扎双侧髂内动脉产生了可靠的创伤性动脉性勃起功能障碍动物模型。动脉结扎几分钟后海绵体内注射VEGF有利于勃起功能的恢复。
Purpose: We tested the hypothesis that intracavernous injection of vascular endothelial growth factor (VEGF) can restore erectile function in a rat model of traumatic arteriogenic erectile dysfunction.Materials and Methods: Exploration of bilateral internal iliac arteries was performed in 50, 3-month-old male rats. A total of 44 rats underwent bilateral ligation of the internal iliac arteries and 6 that underwent exploration only served as the sham operated group. Minutes later intracavernous injection of phosphate buffered saline (PBS) plus bovine serum albumin in 16 rats, 2 mug. VEGF plus PBS plus BSA in 12 and 4 mug. VEGF plus PBS plus BSA in 16 was performed. At weeks 1, 2 and 6 about a third of the rats in each group underwent electrostimulation of the cavernous nerves to assess erectile function and were then sacrificed. Penile tissues were collected for histochemical and electron microscopy examinations.Results: No impairment of erectile function was noted in sham operated rats. Immediately after arterial ligation all rats showed little or no erectile response to neurostimulation. In PBS treated rats modest recovery of erectile function was noted at week 6. Significant recovery of erectile function was noted in VEGF treated rats at weeks 1 and 2 in the 4 mug. group only and at week 6 in the 2 and 4 mug. groups. Neuronal nitric oxide synthase staining showed a reduction in neuronal nitric oxide synthase positive nerve fibers in the dorsal or intracavernous nerves at week 1. Moderate recovery of neuronal nitric oxide synthase positive nerve fibers was noted in the 2 and 4 kg. VEGF treated groups but not in the PBS treated group. Electron microscopy revealed no pathological change in sham operated rats. In dorsal nerves the atrophy of myelinated and nonmyelinated nerve fibers was noted in ligated plus PBS treated rats. Partial recovery was observed in VEGF treated rats. Scattered atrophic smooth muscle cells were seen in PBS and occasionally in VEGF treated rats but not in the sham operated group. The most dramatic findings in VEGF treated rats were hypertrophy and hyperplasia of the endothelial cells, especially those lining the small capillaries.Conclusions: Ligation of bilateral internal iliac arteries produced a reliable animal model of traumatic arteriogenic erectile dysfunction. Intracavernous injection of VEGF minutes after arterial ligation facilitated the recovery of erectile function.