Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity.
Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity.
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Burkholderia Gladioli菌株KJ-34表现出广泛的抗真菌活性。
DOI：
10.3389/fpls.2023.1097044
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发表时间：
2023
影响因子：
5.6
通讯作者：
Zou, Lijuan
中科院分区：
文献类型：
--
作者：
Yang, Chunnan;Wang, Zhihui;Wan, Jiangxue;Qi, Tuo;Zou, Lijuan
植物病原体是全球粮食生产的主要制约因素之一。被鉴定为有效管理植物病原体的微生物的抗生素特性被充分记载。在这里，我们使用拮抗实验和非靶向代谢组学来分离KJ-34产生的潜在抗真菌分子。KJ-34是从水稻根际土壤中分离到的一株生防细菌，对稻曲病菌（Ustilaginoidea virens）、茄链格孢（Alternaria solani）、尖孢镰刀菌（Fusarium oxysporum）、辣椒疫霉（Phytophthora capsica）、玉米棒孢霉（Corynespora acumicola）等多种病原真菌具有较强的拮抗作用。确定了适宜的发酵条件，发酵液处理能显著抑制稻瘟病菌和灰葡萄孢的侵染。发酵液抑制率分别为75%和82%。发酵液处理破坏了孢子萌发，导致菌丝畸形。此外，我们发现，抗真菌产品的分子量小于1000 Da通过半透膜上的固体培养基测定。为了寻找KJ-34产生的潜在抗真菌分子，我们使用质谱优化前后的发酵液进行比较和生物信息学分析。推测非靶向代谢组学分析具有抗真菌剂库，包括苯甲酰星形孢菌素、morellin和东莨菪碱。这些结果表明，KJ-34产生多种生物防治剂，对多种植物病原真菌具有抑制作用，在生态防治技术中具有很强的应用潜力。
Plant pathogens are one of the major constraints on worldwide food production. The antibiotic properties of microbes identified as effective in managing plant pathogens are well documented. Here, we used antagonism experiments and untargeted metabolomics to isolate the potentially antifungal molecules produced by KJ-34. KJ-34 is a potential biocontrol bacterium isolated from the rhizosphere soil of rice and can fight multiple fungal pathogens (i.e. Ustilaginoidea virens, Alternaria solani, Fusarium oxysporum, Phytophthora capsica, Corynespora cassiicola). The favoured fermentation conditions are determined and the fermentation broth treatment can significantly inhibit the infection of Magnaporthe oryzae and Botryis cinerea. The fermentation broth suppression ratio is 75% and 82%, respectively. Fermentation broth treatment disrupted the spore germination and led to malformation of hyphae. Additionally, we found that the molecular weight of antifungal products were less than 1000 Da through semipermeable membranes on solid medium assay. To search the potentially antifungal molecules that produce by KJ-34, we used comparative and bioinformatics analyses of fermentation broth before and after optimization by mass spectrometry. Untargeted metabolomics analyses are presumed to have a library of antifungal agents including benzoylstaurosporine, morellin and scopolamine. These results suggest that KJ-34 produced various biological control agents to suppress multiple phytopathogenic fungi and showed a strong potential in the ecological technologies of prevention and protection.
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影响因子：
5.6
作者：
通讯作者：
--
产生挥发性有机化合物的拮抗木霉的生物防治和植物生长促进特性。
影响因子：
5.6
作者：
通讯作者：
--
影响因子：
7.7
作者：
通讯作者：
--
影响因子：
2.7
作者：
Li D;Oku N;Hasada A;Shimizu M;Igarashi Y
通讯作者：
Igarashi Y
影响因子：
4.4
作者：
Deng, Peng;Foxfire, Adam;Lu, Shi-En
通讯作者：
Lu, Shi-En