Electroacupuncture Alleviates Bladder Overactivity via Inhabiting Bladder P2X3 Receptor
Electroacupuncture Alleviates Bladder Overactivity via Inhabiting Bladder P2X3 Receptor
复制标题
电针通过抑制膀胱 P2X3 受体缓解膀胱过度活动
DOI：
10.1155/2020/4080891
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发表时间：
2020-03-17
影响因子：
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通讯作者：
Chen, Yue-lai
中科院分区：
文献类型：
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作者：
Feng, Qi-fan;Zhang, An-dong;Chen, Yue-lai
电针治疗膀胱过动症已被广泛应用，但其作用机制尚不清楚。本研究旨在探讨EA对大鼠OAB嘌呤能信号通路的调节作用。采用电针（连续波，30 Hz, 1 mA）刺激大鼠纤毛穴（BL32）和回阳穴（BL35）。结果表明，当膀胱外周水平和脊髓中枢水平的P2X3受体参与膀胱排尿的传入信号反射时，静脉给药P2X3拮抗剂AF-353可显著抑制初生大鼠排尿和大鼠OAB，增加膀胱容量和排尿压力。EA刺激可显著缓解膀胱过度活动，阻断P2X3受体后，EA作用减弱。EA刺激可有效降低大鼠OAB、脊髓（L6-S1）和DRG （L6-S1）中P2X3 mRNA和蛋白的表达，显著减少大鼠OAB、脊髓（L6-S1）和DRG （L6-S1）中P2X3阳性细胞的数量。上述结果提示，EA刺激可缓解膀胱过度活动，其功能与膀胱中P2X3受体的存在密切相关。
Electroacupuncture (EA) has been widely applied for overactive bladder, but the mechanism of its action remains to be clarified. This study was aimed to investigate EA regulating the effect of purinergic signaling in the OAB of rats. Electroacupuncture (continuous wave, 30 Hz, 1 mA) was applied to stimulate the Ciliao point (BL32) and the Huiyang point (BL35) of rats. Results showed that when the P2X3 receptor in bladder peripheral level and the spinal cord central level was involved in the bladder micturition reflex of the afferent signaling, intravenous administration P2X3 antagonist AF-353 can significantly inhibit urination in naive rats and OAB of rats and increase bladder volume and micturition pressure. EA stimulation alleviated bladder overactivity significantly and after the P2X3 receptor was blocked, the EA effect was weakened. EA stimulation can effectively reduce the P2X3 mRNA and protein expression in OAB of rats, spinal cord (L6-S1), and DRG (L6-S1) and can significantly reduce the number of positive P2X3 cells in OAB of rats, spinal cord (L6-S1), and DRG (L6-S1). These findings suggest that EA stimulation could alleviate bladder overactivity, and the function is closely related to the inhabited P2X3 receptor in the bladder.