Decrease in luteinizing hormone pulse frequency during a five-hour peripheral ghrelin infusion in the ovariectomized rhesus monkey.
Decrease in luteinizing hormone pulse frequency during a five-hour peripheral ghrelin infusion in the ovariectomized rhesus monkey.
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DOI：
10.1210/jc.2004-1244
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发表时间：
2004-11
期刊：
影响因子：
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通讯作者：
Nicolas R Vulliémoz;E. Xiao;L. Xia-Zhang;M. Germond;J. Rivier;M. Ferin
中科院分区：
文献类型：
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作者：
Nicolas R Vulliémoz;E. Xiao;L. Xia-Zhang;M. Germond;J. Rivier;M. Ferin
胃饥饿素是一种由胃分泌的与营养相关的肽，在长期缺乏食物时会升高。由于营养不良通常与生殖轴受抑制有关，因此我们假设增加外周生长素释放肽水平会降低 GnRH 脉冲发生器的活性。成年切除卵巢的恒河猴 (n = 6) 接受 5 小时静脉注射人生长素释放肽（100 至 150 微克推注，然后 100-150 微克/小时）或盐水输注，然后输注 3 小时盐水以建立基线脉动 LH 释放。在整个实验过程中，每隔 15 分钟采集一次血样。 Ghrelin 输注使血浆 Ghrelin 水平增加至基线的 2.9 倍。 Ghrelin 显着降低 LH 脉冲频率（从基线的 0.89 +/- 0.07/h 降至 ghrelin 输注期间的 0.57 +/- 0.10/h；P < 0.05，平均值 +/- sem），而在盐水治疗期间 LH 脉冲频率保持不变。 LH 脉冲幅度不受影响。生长素释放肽还显着刺激皮质醇和生长激素的释放，但对瘦素没有影响。我们得出的结论是，生长素释放肽可以抑制 GnRH 脉冲活性，从而可能介导在营养不良（例如神经性厌食症）条件下观察到的生殖系统抑制。 Ghrelin 还激活肾上腺轴，但其与抑制 GnRH 脉冲频率的相关性仍有待确定。
Ghrelin, a nutrition-related peptide secreted by the stomach, is elevated during prolonged food deprivation. Because undernutrition is often associated with a suppressed reproductive axis, we have postulated that increasing peripheral ghrelin levels will decrease the activity of the GnRH pulse generator. Adult ovariectomized rhesus monkeys (n = 6) were subjected to a 5-h iv human ghrelin (100- to 150-microg bolus followed by 100-150 microg/h) or saline infusion, preceded by a 3-h saline infusion to establish baseline pulsatile LH release. Blood samples were collected at 15-min intervals throughout the experiment. Ghrelin infusion increased plasma ghrelin levels 2.9-fold of baseline. Ghrelin significantly decreased LH pulse frequency (from 0.89 +/- 0.07/h in baseline to 0.57 +/- 0.10/h during ghrelin infusion; P < 0.05, mean +/- sem), whereas LH pulse frequency remained unchanged during saline treatment. LH pulse amplitude was not affected. Ghrelin also significantly stimulated both cortisol and GH release, but had no effect on leptin. We conclude that ghrelin can inhibit GnRH pulse activity and may thereby mediate the suppression of the reproductive system observed in conditions of undernutrition, such as in anorexia nervosa. Ghrelin also activates the adrenal axis, but the relevance of this to the inhibition of GnRH pulse frequency remains to be established.