Multi-tier regulation of the streptomycete morphogenetic peptide SapB
Multi-tier regulation of the streptomycete morphogenetic peptide SapB
复制标题
DOI：
10.1111/j.1365-2958.2012.08041.x
复制
发表时间：
2012-05-01
影响因子：
3.6
通讯作者：
Willey, Joanne M.
中科院分区：
文献类型：
--
作者：
Gaskell, Alisa A.;Giovinazzo, Joseph A.;Willey, Joanne M.
天蓝色链霉菌是一种形态复杂的细菌，需要分泌表面活性蛋白来完成其生命周期。SapB代表了一类重要的生物表面活性剂，如其能够恢复气生菌丝形成时，外源施加到发育突变体。然而，这种气生菌丝未能形成孢子，这说明需要协调SapB生产与其他发育事件的时间。SapB具有不寻常的lantibiotic结构。其结构基因ramS仅位于编码其假定修饰酶RamC的基因下游38个核苷酸。操纵子的瞬时协调表达被认为是由反应调节因子RamR控制的。然而，我们发现，ramS转录在整个细胞周期的双重表达谱不同的严格控制ramC的表达。令人惊讶的是，翻译后修饰依赖于前体肽RamS的先前膜定位，如S中不存在RamS修饰所证明的。用枯草芽孢杆菌脂蛋白表面活性素处理的天蓝色菌丝。我们的研究结果表明，物种间的相互作用也可以介导的干扰翻译后事件。此外，时间和空间调节的不可逆的翻译后修饰的表面活性的形态发生肽提出了一个新的模式控制的关键发展的事件。
Streptomyces coelicolor is a morphologically complex bacterium requiring the secretion of surface-active proteins to progress through its life cycle. SapB represents an important class of these biosurfactants, as illustrated by its ability to restore aerial hyphae formation when applied exogenously to developmental mutants. However, such aerial hyphae fail to sporulate, exemplifying the need to co-ordinate the timing of SapB production with other developmental events. SapB has an unusual lantibiotic structure. Its structural gene, ramS, is only 38 nucleotides downstream of the gene encoding its putative modification enzyme, RamC. Transient, co-ordinated expression of the operon was thought to be controlled by the response regulator RamR. However, we show that ramS is transcribed throughout the cell cycle with a dual expression profile dissimilar to the tightly controlled ramC expression. Surprisingly, post-translational modification relies on prior membrane localization of the precursor peptide, RamS, as demonstrated by the absence of RamS modification in S. coelicolor hyphae treated with the Bacillus subtilis lipoprotein surfactin. Our results demonstrate that interspecies interaction can also be mediated by interference of post-translational events. Further, temporal and spatial regulation of irreversible post-translational modification of a surface-active morphogenetic peptide suggests a new model for the control of key developmental events.