Histological study on seminal plasma absorption and spermiophagy in the epididymal region of domestic fowl.
Histological study on seminal plasma absorption and spermiophagy in the epididymal region of domestic fowl.
复制标题
家禽附睾区精浆吸收和自噬的组织学研究。
作者：
M. Nakai;Y. Hashimoto;H. Kitagawa;Y. Kon;N. Kudo
本文用超微结构和组织化学方法研究了鸡附睾区精浆的吸收和精子的吞噬。睾丸网上皮细胞有稀疏的被膜囊泡，很少出现噬精子现象。睾丸网腔内有许多巨噬细胞活跃地吞噬精子。近端输出小管中的无纤毛细胞具有发达的微绒毛、包被小泡、大量管状结构和顶端细胞质中的溶酶体。它们很少含有精子碎片。在这些细胞的腔边界处观察到强烈的碱性磷酸酶活性。纤毛细胞没有特征表明积极吸收精浆。连接小管和附睾管上皮细胞有大量微绒毛，少量小泡和小溶酶体。它们不含精子。在连接小管和附睾管上皮细胞的管腔和侧面观察到强烈的酸性磷酸酶活性。将辣根过氧化物酶注入传出小管后，在近端传出小管无纤毛细胞的囊泡和管状结构中检测到大量的反应产物。这些结果表明，精浆的吸收主要发生在输出小管，巨噬细胞的噬精作用发生在鸡附睾区的睾丸网。
Absorption of seminal plasma and spermiophagy in the fowl epididymal region were studied ultrastructurally and histochemically. Epithelial cells of the rete testis had sparse coated vesicles and rarely showed spermiophagy. Many macrophages in the lumen of the rete testis actively phagocytosed spermatozoa. Nonciliated cells in the proximal efferent ductules had well-developed microvilli, coated vesicles, numerous tubular structures, and lysosomes in their apical cytoplasm. They rarely contained fragments of spermatozoa. Intense alkaline phosphatase activity was observed at the luminal borders of these cells. Ciliated cells had no features indicating active absorption of seminal plasma. Epithelial cells of the connecting ductules and epididymal duct had numerous microvilli, a few vesicles, and small lysosomes. They did not contain spermatozoa. Intense acid phosphatase activity was observed on the luminal and lateral surfaces of the epithelial cells of the connecting ductules and epididymal duct. After injection of horseradish peroxidase into the excurrent ducts, a large amount of reaction product was detected in the vesicles and tubular structures of the nonciliated cells of the proximal efferent ductules. These results suggest that the absorption of seminal plasma occurred mainly in the efferent ductules, and that spermiophagy by macrophages occurred in the rete testis in the fowl epididymal region.