Expression of bone morphogenetic protein-2 via adenoviral vector in C2C12 myoblasts induces differentiation into the osteoblast lineage.
Expression of bone morphogenetic protein-2 via adenoviral vector in C2C12 myoblasts induces differentiation into the osteoblast lineage.
复制标题
通过腺病毒载体在 C2C12 成肌细胞中表达骨形态发生蛋白 2，诱导分化为成骨细胞谱系。
DOI：
10.1006/bbrc.1999.1281
复制
发表时间：
1999
影响因子：
3.1
通讯作者：
S. Miyatake
中科院分区：
文献类型：
--
作者：
Y. Okubo;K. Bessho;K. Fujimura;Tadahiko Iizuka;S. Miyatake
为了研究骨形态发生蛋白（BMP）-2基因转移到C2 C12成肌细胞的有效性，我们构建了一个人BMP-2表达的复制缺陷型腺病毒载体AxCAOBMP-2。用该病毒载体或表达大肠杆菌LacZ基因的对照腺病毒载体在体外感染C2 C12细胞。通过X-gal染色证实了用LacZ基因表达载体向C2 C12细胞的有效基因转移。通过免疫荧光和Western印迹分析证实了用该病毒载体感染的C2 C12细胞中BMP-2的丰富表达。通过该载体转移BMP-2基因的C2 C12细胞在细胞中产生碱性磷酸酶，并且还在培养基中产生并分泌骨钙素，这表明在体外将BMP-2基因转移到C2 C12细胞中可以将这些细胞从成肌细胞转变成成骨细胞谱系。
To examine the effectiveness of a gene transfer of bone morphogenetic protein (BMP)-2 into C2C12 myoblasts, we constructed a human BMP-2-expressing replication-deficient adenoviral vector, AxCAOBMP-2. C2C12 cells were infected in vitro with either this viral vector or an Escherichia coli LacZ gene-expressing control adenovirus vector. An efficient gene transfer to the C2C12 cells was confirmed with the LacZ gene-expressing vector by X-gal staining. Abundant BMP-2 expression in C2C12 cells infected with this viral vector was confirmed by immunofluorescence and Western blot analysis. C2C12 cells transferred with the BMP-2 gene by this vector produced alkaline phosphatase in the cells and also produced and secreted osteocalcin in the culture medium, demonstrating that a gene transfer of BMP-2 into C2C12 cells in vitro could convert these cells from myoblast to osteoblast lineage.
影响因子：
13
作者：
Reddi, A. Hari
通讯作者：
Reddi, A. Hari
影响因子：
4.1
作者：
Musgrave, DS;Bosch, P;Huard, J
通讯作者：
Huard, J