Impaired Myocardium Regeneration With Skeletal Cell Sheets-A Preclinical Trial for Tissue-Engineered Regeneration Therapy
Impaired Myocardium Regeneration With Skeletal Cell Sheets-A Preclinical Trial for Tissue-Engineered Regeneration Therapy
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DOI：
10.1097/tp.0b013e3181e6f201
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发表时间：
2010-08-27
期刊：
影响因子：
6.2
通讯作者：
Sawa, Yoshiki
中科院分区：
文献类型：
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作者：
Miyagawa, Shigeru;Saito, Atsuhiro;Sawa, Yoshiki
背景。我们假设自体骨细胞（SC）片再生猪心脏梗死心肌作为临床前试验。方法与结果。在左前降部植入ameroid缩窄器4周后造心。将从腿部肌肉中分离的SCs培养，并在20℃下作为单层细胞片从温度响应结构域包被培养皿中分离。假手术（C组n=5）。超声心动图显示，SC组术后3个月和6个月心脏功能明显改善(分数面积缩短，3个月；SC vs. C=49.5+/-2.8 vs. 24.6+/-2.0%， P
Background. We hypothesized that autologous skeletal cell (SC) sheets regenerate the infract myocardium in porcine heart as a preclinical trial.Methods and Results. The impaired heart was created by implantation of ameroid constrictor on left anterior descending for 4 weeks. SCs isolated from leg muscle were cultured and detached from the temperature-responsive domain-coated dishes as single monolayer cell sheet at 20 degrees C. The following therapies were conducted: SC sheets (SC group, n=5); sham (C group n=5). Echocardiography demonstrated that cardiac performance was significantly improved in the SC group 3 and 6 months after operation (fractional area shortening, 3 months; SC vs. C=49.5+/-2.8 vs. 24.6+/-2.0%, P