Quantitation of antigen-specific immune responses in human immunodeficiency virus (HIV)-infected individuals by limiting dilution analysis.
Quantitation of antigen-specific immune responses in human immunodeficiency virus (HIV)-infected individuals by limiting dilution analysis.
复制标题
通过有限稀释分析对人类免疫缺陷病毒 (HIV) 感染者的抗原特异性免疫反应进行定量。
DOI：
10.1007/bf00918092
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发表时间：
1992
影响因子：
9.1
通讯作者：
Whitacre,CC
中科院分区：
文献类型：
--
作者：
Sabbaj,S;Para,MF;Fass,RJ;Adams,PW;Orosz,CG;Whitacre,CC
HIV感染后，淋巴细胞对Recall抗原的增殖反应丧失。我们试图设计一种方法，通过这种方法可以定量监测艾滋病毒感染早期人群的功能免疫状态。用常规淋巴细胞增殖试验和有限稀释法检测了45例HIV感染者和11例对照组对破伤风类毒素的反应，并用白细胞介素2的分泌作为反应的量度。我们的数据显示，有限稀释法在80%的受试者中检测到破伤风类毒素反应性T细胞，而通过增殖检测，这一比例仅为44%。然而，HIV感染者的破伤风反应性T细胞频率(中位数为1/59,156)比血清阴性对照组(中位数为1/11,599)降低了五倍。纵向研究表明，在HIV感染者中，破伤风特异性T细胞反应的频率随时间而降低。因此，极限稀释法是检测HIV感染者中抗原特异性T细胞的一种定量方法，并可用于监测HIV感染时T细胞功能的变化。
The lymphocyte proliferative response to recall antigens is lost following HIV infection. We sought to devise a means by which the functional immune status of persons in the early stages of HIV infection could be monitored quantitatively. The response to tetanus toxoid was examined in 45 HIV-infected individuals and 11 controls using conventional lymphocyte proliferative assays concurrently with limiting dilution analysis utilizing the secretion of interleukin-2 as the measure of a response. Our data show that the limiting dilution analysis detects tetanus toxoid-reactive T cells in 80% of those tested, as compared to only 44% by proliferation. However, the frequency of tetanus-reactive T cells in HIV-infected individuals (median frequency = 1/59,156) is decrease five-fold as compared to seronegative controls (median frequency = 1/11,599). Longitudinal studies demonstrated a time-dependent decrease in the frequency of tetanus-specific T cell responses in the HIV-infected individuals. Thus, the limiting dilution analysis is a quantitative approach for detecting antigen-specific T cells in HIV-infected individuals, and may be used to monitor changes in T cell function in HIV infection.