A novel locus essential for spreading of Cytophaga hutchinsonii colonies on agar
A novel locus essential for spreading of Cytophaga hutchinsonii colonies on agar
复制标题
哈钦森噬细胞菌菌落在琼脂上传播所必需的新位点
DOI：
10.1007/s00253-013-4820-2
复制
发表时间：
2013-08-01
影响因子：
5
通讯作者：
Lu, Xuemei
中科院分区：
文献类型：
--
作者：
Ji, Xiaofei;Bai, Xinfeng;Lu, Xuemei
Cytophaga hutchinsonii是一种需氧纤维素分解滑行细菌。其细胞在没有鞭毛和IV型皮利的表面上运动的机制尚不清楚。在这项研究中，基于mariner的转座子诱变，以确定一个新的位点CHU_1797所必需的菌落传播在硬和软琼脂表面通过滑动。CHU_1797编码一个348个氨基酸的外膜蛋白，其功能未知，与CHU_1797具有高度序列相似性的蛋白广泛存在于拟杆菌门的成员中。CHU_1797的破坏抑制了琼脂表面上向葡萄糖的扩散，但对已经与纤维素接触的细胞的纤维素降解没有显著影响。扫描电镜观察表明，突变体细胞在纤维素纤维表面的排列也与野生型相似。这些结果表明，纤维素降解不需要殖民地的扩展能力。哈钦森氏菌这是首次对C.哈钦森氏菌
Cytophaga hutchinsonii is an aerobic cellulolytic gliding bacterium. The mechanism of its cell motility over surfaces without flagella and type IV pili is not known. In this study, mariner-based transposon mutagenesis was used to identify a new locus CHU_1797 essential for colony spreading on both hard and soft agar surfaces through gliding. CHU_1797 encodes a putative outer membrane protein of 348 amino acids with unknown function, and proteins which have high sequence similarity to CHU_1797 were widespread in the members of the phylum Bacteroidetes. The disruption of CHU_1797 suppressed spreading toward glucose on an agar surface, but had no significant effect on cellulose degradation for cells already in contact with cellulose. SEM observation showed that the mutant cells also regularly arranged on the surface of cellulose fiber similar with that of the wild type strain. These results indicated that the colony spreading ability on agar surfaces was not required for cellulose degradation by C. hutchinsonii. This was the first study focused on the relationship between cell motility and cellulose degradation of C. hutchinsonii.