Fabrication of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays for enhancing bactericidal effect and osteoinductivity
Fabrication of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays for enhancing bactericidal effect and osteoinductivity
复制标题
制备银纳米粒子掺杂羟基磷灰石定向块阵列涂层以增强杀菌效果和骨诱导性
DOI：
10.1016/j.jmbbm.2016.04.002
复制
发表时间：
2016
影响因子：
3.9
通讯作者：
Zhu Zhenan
中科院分区：
文献类型：
--
作者：
Tian Bo;Chen Wei;Yu Degang;Lei Yong;Ke Qinfei;Guo Yaping;Zhu Zhenan
植入物相关感染是骨科手术中常见的术后并发症，也是骨科手术中的一个严重问题。本工作描述了银纳米粒子掺杂的羟基磷灰石涂层与定向块阵列（AgNP-BHAC）的合成。使用扫描电子显微镜、能量色散光谱法、透射电子显微镜、X射线粉末衍射和傅里叶变换红外光谱法研究了所得纳米结构。AgNP-BHAC对革兰氏阴性大肠杆菌和革兰氏阳性金黄色葡萄球菌表现出良好的抗菌活性，这是由于银纳米颗粒的抗菌作用。人骨髓基质细胞（hBMSC）培养显示，AgNP-BHAC表现出更好的生物相容性，并允许改善细胞增殖，附着，和骨诱导性比未涂层的Ti-6Al-4V钛合金，生物医学应用的青睐材料。综上所述，本研究提供了一种方便有效的方法，将银掺入到具有块状形貌的HA涂层中。该方法可用于修饰各种金属植入物表面，以提高其抗菌效果并降低潜在的长期细胞毒性。
Implant-associated infection is a common postoperative complication and remains a serious problem in orthopedic surgery. This work describes the synthesis of silver nanoparticle-doped hydroxyapatite coatings with oriented block arrays (AgNP-BHAC). The resulting nanostructure was investigated using scanning electron microscopy, energy-dispersive spectrometry, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy. AgNP-BHAC exhibited excellent antimicrobial activity toward gram-negativeEscherichia coliand gram-positiveStaphylococcus aureusowing to the antibacterial effects of the silver nanoparticles. Human bone marrow stromal cells (hBMSC) culture revealed that the AgNP-BHAC exhibited better biocompatibility, and permitted improved cell proliferation, attachment, and osteoinductivity than uncoated Ti–6Al–4V titanium alloy, the favored material for biomedical applications. In summary, this study presents a convenient and effective method for the incorporation of silver into HA coatings with block morphology. This method can be utilized to modify a variety of metallic implant surfaces to improve their antimicrobial effects and reduce potential long-term cytotoxicity.