The heterologous expression in Arabidopsis of a chrysanthemum Cys2/His2 zinc finger protein gene confers salinity and drought tolerance
The heterologous expression in Arabidopsis of a chrysanthemum Cys2/His2 zinc finger protein gene confers salinity and drought tolerance
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菊花Cys2/His2锌指蛋白基因在拟南芥中的异源表达赋予耐盐性和耐旱性
DOI：
10.1007/s00425-011-1558-x
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发表时间：
2012-05-01
期刊：
影响因子：
4.3
通讯作者：
Chen, Sumei
中科院分区：
文献类型：
--
作者：
Gao, Haishun;Song, Aiping;Chen, Sumei
锌指蛋白（ZFP）在植物生长发育中起着重要作用。利用RACE PCR技术从菊花中克隆了CgZFP 1基因，该基因包含两个保守的C2H2区，即核定位区（B box）、富含Leu区（L box）和保守的DLN区（DLN box）。它在菊花叶片中的表达受盐和干旱的强烈诱导，但不受阿坝的诱导。亚细胞定位实验表明CgZFP 1蛋白在体内定位于细胞核。酵母单杂交试验表明，CgZFP 1具有转录激活能力，异源表达CgZFP 1可使转基因拟南芥同时耐受盐和干旱胁迫。在盐胁迫下，与渗透调节、活性氧清除和离子稳态相关的基因AtLea3、AtP5CS2、AtProT1和AtMnSOD、AtPOD、AtAPX1、AtSOS1、AtSOS2、AtSOS3、AtNHX 1在转CgZFP 1基因拟南芥中表达增强。此外，在干旱胁迫下，与渗透调节和氧化胁迫相关的基因Atlea3、AtP5CS2、AtProT1、水通道蛋白AtPIP2A和AtMnSOD、AtPOD、AtAPX1在转CgZFP 1基因拟南芥中均被诱导表达。这些结果表明CgZFP 1是植物盐和干旱胁迫反应中的一个重要调节因子。
Zinc finger proteins (ZFPs) play an important role in plant growth and development. Here, we describe the isolation ofCgZFP1, a Cys2/His2 (C2H2) ZFP gene, using RACE PCR from chrysanthemum, and the investigation of its function with ectopic expression in Arabidopsis.CgZFP1contains two conserved C2H2regions, a nuclear localization domain (B box), a Leu-rich domain (L box) and a conserved DLN sequence (DLN box) close to its C-terminus. Its expression in the chrysanthemum leaf was strongly induced by salinity or drought, but not by ABA. Subcellular localization assay indicated that CgZFP1 protein is localized in nucleus in vivo. Yeast-one hybrid assay showed thatCgZFP1possesses transcriptional activation ability, heterologous expression ofCgZFP1conferred tolerance of transgenic Arabidopsis plants to both salinity and drought stresses. Under salinity stress, genes involved in osmotic adjustment, ROS scavenging, and ion homeostasis:Atlea3,AtP5CS2,AtProT1, andAtMnSOD,AtPOD,AtAPX1, andAtSOS1,AtSOS2,AtSOS3,AtNHX1were enhanced inCgZFP1transgenic Arabidopsis plants. Moreover, genes involved in the osmotic adjustment and oxidative stress responses:Atlea3,AtP5CS2,AtProT1, the aquaporinAtPIP2A, andAtMnSOD,AtPOD,AtAPX1were induced inCgZFP1transgenic Arabidopsis under drought stress. These results indicateCgZFP1is an important regulator involved in the salt and drought stress response in plants.