A novel strategy for MALDI-TOF MS analysis of small molecules
A novel strategy for MALDI-TOF MS analysis of small molecules
复制标题
DOI：
10.1016/j.jasms.2009.09.024
复制
发表时间：
2010
影响因子：
3.2
通讯作者：
Shu Zhang;Jian’an Liu;Yi Chen;S. Xiong;Guanghui Wang;Jun Chen;Guoqiang Yang
中科院分区：
文献类型：
--
作者：
Shu Zhang;Jian’an Liu;Yi Chen;S. Xiong;Guanghui Wang;Jun Chen;Guoqiang Yang
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）对小分子（通常分子量低于500 Da）的分析效率不高，因为低m/z区域的基质相关峰会产生干扰。针对这个问题开发的先前方法集中于减少由传统矩阵引起的峰值。在这里，我们报告了一种新的策略，分析小分子在一个高的和无干扰的质量范围内，通过使用金属酞菁（MPCS）作为矩阵，应该能够形成矩阵分析物加合物。通过从MPc-分析物加合物的质量中减去MPc的质量，计算目标分析物的质量。MPCs也是可检测的，可以作为内标。然后合成并探索了具有芳香族或脂肪族基团和不同金属中心的各种MPcs。铝酞菁（AlPcs），镓酞菁（GaPcs），铟酞菁（InPcs）是有效的矩阵，形成MPc分析物加合物的正离子或负离子模式。检测限从17到75 fmol不等，这取决于分析物的类型。并对加合物的形成机理进行了探讨。总的来说，我们的策略提供了一种通过MALDI-TOF MS分析小分子的新型有效方法。
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) does not work efficiently on small molecules (usually with molecular weight below 500 Da) because of the interference of matrix-related peaks in low m/z region. The previous methods developed for this problem focused on reducing the peaks caused by the traditional matrices. Here, we report a novel strategy to analyze small molecules in a high and interference-free mass range by using metal-phthalocyanines (MPcs) as matrices which should be capable of forming matrix-analyte adducts. The mass of the target analyte was calculated by subtracting the mass of MPc from the mass of the MPc–analyte adduct. MPcs were also detectable and could serve as internal standards. Various MPcs with aromatic or aliphatic groups and different metal centers were then synthesized and explored. Aluminum-phthalocyanines (AlPcs), gallium-phthalocyanines (GaPcs), and indium-phthalocyanines (InPcs) were efficient matrices to form MPc–analyte adducts in either the positive or negative ion mode. The detection limits varied from 17 to 75 fmol, depending on analyte types. The mechanism of adducts formation was also proposed. Collectively, our strategy provides a novel and efficient way to analyze small molecules by MALDI-TOF MS.