Activation of endogenous retroviral sequences in human leukemia.
Activation of endogenous retroviral sequences in human leukemia.
复制标题
人类白血病中内源性逆转录病毒序列的激活。
DOI：
10.1016/0006-291x(85)91227-6
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Wilkowski,C
中科院分区：
文献类型：
--
作者：
McClain,K;Wilkowski,C
内源性逆转录病毒序列已经在包括人类在内的几个物种的基因组中被鉴定。在人类各种恶性细胞表面发现了与灵长类内源性病毒相似的蛋白质，为了进一步确定这种作用，我们使用狒狒内源性病毒的灵长类逆转录病毒DNA探测人类基因组文库中的相关序列。在低严格性杂交条件下，共分离到45个与BaEV gag-pol同源的克隆。其中，有几个被发现含有DNA，其在人类淋巴白血病细胞中比在正常淋巴细胞中以更高水平表达为RNA。
Endogenous retroviral sequences have been identified in the genomes of several species including humans. Proteins similar to those of primate endogenous viruses have been found on the surface of various malignant cells in man. To further define this role, we have used a primate retrovirus DNA from the Baboon Endogenous Virus to probe a human genomic library for related sequences. A total of 45 clones homologous to BaEV gag-pol were isolated under low stringency hybridization. Of these, several were found to contain DNA which was expressed as RNA at higher levels in human lymphoid leukemic cells than in normal lymphocytes.