Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow.
Selective expression of CD45 isoforms on functional subpopulations of CD34+ hemopoietic cells from human bone marrow.
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DOI：
10.1084/jem.172.1.363
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发表时间：
1990-07-01
期刊：
影响因子：
--
通讯作者：
Eaves CJ
中科院分区：
文献类型：
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作者：
Lansdorp PM;Sutherland HJ;Eaves CJ
我们发现，人类骨髓中以表达CD34为特征的一小部分细胞可以很容易地细分为两个明显不重叠的大小大致相同的亚群，一个表达CD45RO，一个表达CD45R。对这些亚群的功能研究表明，原始红系集落形成细胞(BFU-E)均为CD34+CD45RO+。同样，在融合照射的长期骨髓培养饲养层上培养5wk后同时产生红系和粒系集落形成细胞的更多原始细胞也显示出这种表型。而大多数粒系集落形成细胞为CD34+CD45RO-细胞。CD45亚型在不同功能亚群的造血细胞上的差异表达与这些分子在原始的、通常静止的造血细胞的分化或激活中发挥重要作用的概念是一致的。在长期骨髓培养系统中，CD45RO的存在和人类细胞上CD45R的缺乏与啮齿动物可移植的造血干细胞上CD45R的缺乏相一致，这可能是对人类干细胞纯化或CD45R+白血病细胞净化策略的有益补充。
We have found that the small population of cells in human marrow that are characterized by their expression of CD34 can be readily subdivided into two apparently nonoverlapping subpopulations of approximate equal size, one expressing CD45RO and one CD45R. Functional studies of these subpopulations revealed that all of the primitive erythroid colony- forming cells (BFU-E) are CD34+ CD45RO+. Similarly, more primitive cells that give rise to both erythroid and granulopoietic colony- forming cells after being maintained for 5 wk on confluent irradiated long-term marrow culture feeder layers, also show this phenotype. In contrast, most granulopoietic colony-forming cells are CD34+ CD45RO- cells. The differential expression of CD45 isoforms on distinct functional subpopulations of hemopoietic cells is consistent with the concept that these molecules play an important role in the differentiation or activation of primitive, normally quiescent, hemopoietic cells. The presence of CD45RO and the lack of CD45R on human cells capable of initiating hemopoiesis in the long-term marrow culture system correspond to the reported lack of CD45R on transplantable hemopoietic stem cells in rodents and may be a useful addition to strategies for human stem cell purification, or for purging CD45R+ leukemic cells.