Productive nonlytic human immunodeficiency virus type 1 replication in a newly established human leukemia cell line.
Productive nonlytic human immunodeficiency virus type 1 replication in a newly established human leukemia cell line.
复制标题
生产性非裂解性人类免疫缺陷病毒 1 型在新建立的人类白血病细胞系中复制。
DOI：
10.1073/pnas.89.21.9996
复制
发表时间：
1992
影响因子：
11.1
通讯作者：
Acs,G
中科院分区：
文献类型：
--
作者：
Banerjee,R;Bekesi,JG;Tarcsafalvi,A;Sperber,K;Deak,G;Choi,HS;Paronetto,F;Holland,JF;Acs,G
我们从慢性粒单核细胞白血病患者的胸膜渗出液中分离出一种淋巴样细胞系MDS。这些细胞是双表型的，含有各种T细胞和骨髓标志物，并且表面对CD 4和CD 8呈阴性，但具有低的CD 4 mRNA。细胞在悬浮液中生长，倍增时间为15小时，核型分析为21三体，对人类免疫缺陷病毒1型（HIV-1）呈阴性，在裸鼠中具有致瘤性。我们用pHXBc 2转染MDS细胞，分离出两株稳定的HIV-1产生细胞系，MDS-T和MDS-I，用HIV-1 IIIB感染MDS细胞。在24小时内，1 × 105个MDS-T或MDS-I细胞产生46 ng p24/ml和能够将0.2 pmol [32 P]TTP掺入oligo（dT）.poly（A）的逆转录酶。超微结构研究显示，MDS-T和MDS-I细胞中存在大量能够感染T细胞的成熟病毒颗粒。抗CD 4抗体Leu 3a可抑制MDS细胞中HIV-1的感染，抑制率为25%。一年多来，MDS-T和MDS-I细胞一直在培养中产生高浓度的HIV-1。当用HIV-1转染或感染时，源自MDS细胞的亚克隆表现得像亲本细胞。与其他T细胞系相反，佛波醇12-肉豆蔻酸酯13-乙酸酯和肿瘤坏死因子α均不刺激HIV-1的复制，而溴腺苷3 '，5'-环一磷酸或干扰素α分别对逆转录酶的产生50%和80%的抑制。这些慢性感染的T细胞系是研究抗HIV药物和HIV-1复制所需细胞因子的作用的有用模型系统。
We have isolated a lymphoid cell line, MDS, from the pleural exudate of a patient with chronic myelomonocytic leukemia. The cells are biphenotypic, containing various T-cell and myeloid markers, and are surface negative for CD4 and CD8 but have low CD4 mRNA. The cells grow in suspension with a doubling time of 15 hr, have been karyotyped as trisomy 21, are negative for human immunodeficiency virus type 1 (HIV-1), and are tumorigenic in the nude mouse. We have isolated two stable HIV-1-producing cell lines, MDS-T, by transfecting MDS cells with pHXBc2, and MDS-I, by infecting MDS cells with HIV-1IIIB. In 24 hr, 1 x 10(5) MDS-T or MDS-I cells produce 46 ng of p24 per ml and reverse transcriptase that is capable of incorporating 0.2 pmol of [32P]TTP into oligo(dT).poly(A). Ultrastructural studies showed numerous mature viral particles in MDS-T and MDS-I cells that are capable of infecting T cells. HIV-1 infection could be inhibited by 25% in the MDS cells with the anti-CD4 antibody Leu 3a. For over a year MDS-T and MDS-I cells have been producing high concentrations of HIV-1 in culture. A subclone derived from the MDS cells behaves like the parent cells when transfected or infected with HIV-1. In contrast to other T-cell lines, neither phorbol 12-myristate 13-acetate nor tumor necrosis factor alpha stimulated the replication of HIV-1, whereas bromoadenosine 3',5'-cyclic monophosphate or interferon alpha caused 50% and 80% inhibition of reverse transcriptase production, respectively. These chronically infected T-cell lines are a useful model system to study the effect of anti-HIV agents and cellular factors required for HIV-1 replication.