Expression of Fc mu receptors on human natural killer cells.
Expression of Fc mu receptors on human natural killer cells.
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Fc mu 受体在人类自然杀伤细胞上的表达。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
T. Whiteside
中科院分区：
文献类型：
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作者：
L. Pricop;C. Galatiuc;M. Manciulea;A. Deleo;A. Sulica;R. Herberman;T. Whiteside
IgG (CD16) Fc受体被认为是大颗粒淋巴细胞（LGL）上唯一的一类免疫球蛋白受体。然而，自然杀伤细胞（NK）在单克隆或多克隆IgM存在下介导抗体依赖性细胞毒性（ADCC）的能力，以及高纯度IgM对NK活性的抑制，不能用IgG的FcR来解释。为了直接评估Fc受体对IgM （Fc mu R）的表达，我们用人多克隆IgM处理NK细胞，并通过直接抗球蛋白玫瑰花结法观察其结合情况，在giemsa染色片上鉴定玫瑰花结形成的LGL。数据显示，高比例的LGL（高达68%）是Fc mu r阳性细胞。然而，这一百分比取决于IgM制剂（多克隆或单克隆）、用于花环测定的指示剂和所研究的细胞制剂。人非贴壁淋巴细胞制剂的双色流式细胞术证实CD56+IgM+细胞的存在，占CD56+细胞的43% ~ 78%。流式细胞术还使用高度富集的人NK细胞制剂（CD3-CD56+细胞的平均百分比为84%）。高达88%的纯化NK细胞以饱和浓度结合fitc标记的单克隆IgM。通过间接免疫荧光，从正常供体外周血中纯化的NK细胞中有34% ~ 62%能够结合多克隆IgM。NK淋巴细胞增生性疾病患者的LGL也获得了类似的结果。因此，通过不同的技术，使用未分离的外周血单核白细胞、纯化的正常NK细胞和NK淋巴细胞增生性疾病患者的LGL，证明了Fc mu R在大多数人类NK细胞上的存在。
Fc receptors for IgG (CD16) have been described as the only type of immunoglobulin receptor on large granular lymphocytes (LGL). However, the ability of natural killer (NK) cells to mediate antibody-dependent cellular cytotoxicity (ADCC) in the presence of monoclonal or polyclonal IgM and the inhibition of NK activity by highly purified IgM could not be explained on the basis of FcR for IgG. In order to directly assess the expression of Fc receptors for IgM (Fc mu R), NK cells were treated with human polyclonal IgM, and its binding was visualized by a direct anti-globulin rosette assay with identification of rosette-forming LGL on Giemsa-stained smears. The data indicated that a high proportion of LGL (up to 68%) were Fc mu R-positive cells. However, this percentage varied depending on the IgM preparation (polyclonal or monoclonal), the indicator reagent used for the rosette assays, and the cell preparations studied. Two-color flow cytometry of human nonadherent lymphocyte preparations confirmed the presence of CD56+IgM+ cells, which represented from 43 to 78% of CD56+ cells. Flow cytometry was also performed using highly enriched preparations of human NK cells (the mean percentage of CD3-CD56+ cells was 84%). Up to 88% of purified NK cells bound FITC-labeled monoclonal IgM at a saturating concentration. By indirect immunofluorescence, from 34 to 62% of NK cells purified from the peripheral blood of normal donors were able to bind polyclonal IgM. Similar results were obtained with LGL from a patient with NK lymphoproliferative disease. Thus the presence of Fc mu R on a majority of human NK cells was demonstrated by different techniques, using unseparated peripheral blood mononuclear leukocytes, purified normal NK cells, and also LGL from a patient with NK lymphoproliferative disease.