Non-specific, on-probe cleanup methods for MALDI-MS samples.
Non-specific, on-probe cleanup methods for MALDI-MS samples.
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DOI：
10.1002/mas.10064
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发表时间：
2003-11
影响因子：
6.6
通讯作者：
Yingda Xu;M. Bruening;J. Watson
中科院分区：
文献类型：
--
作者：
Yingda Xu;M. Bruening;J. Watson
高浓度的污染物如盐和表面活性剂通常存在于生物样品中以溶解或稳定分析物如蛋白质。不幸的是，这些污染物的存在往往排除了直接分析MALDI-MS。选择性吸附的分析物直接修饰的MALDI探针，然后通过冲洗去除污染物，克服了这个问题。本文综述了MALDI探针的各种修饰，使蛋白质和DNA的吸附，即使在大量过量的盐或表面活性剂。沉积在MALDI探针上的用于吸附分析物的界面包括商业聚合物膜、基质晶体薄层、自组装单层膜和聚合物薄膜。疏水和离子相互作用都影响分析物在这些界面上的吸附，并且图案化界面允许分析物分子的浓缩和纯化。
High concentrations of contaminants such as salts and surfactants are often present in biological samples to solubilize or stabilize analytes such as proteins. Unfortunately, the presence of those contaminants often precludes direct analysis by MALDI-MS. Selective adsorption of analytes directly on modified MALDI probes, followed by rinsing to remove contaminants, overcomes this problem. This review focuses on various modifications of MALDI probes to allow the adsorption of proteins and DNA, even in a large excess of salt or surfactant. Interfaces deposited on the MALDI probes to adsorb analytes include films of commercial polymers, thin layers of matrix crystals, self-assembled monolayers, and ultrathin polymer films. Hydrophobic and ionic interactions both effect analyte adsorption on those interfaces, and patterned interfaces allow the concentration and purification of analyte molecules.