Comparative Analysis of H&E and Prussian Blue Staining in a Mouse Model of Cerebral Microbleeds
Comparative Analysis of H&E and Prussian Blue Staining in a Mouse Model of Cerebral Microbleeds
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DOI：
10.1369/0022155414546692
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发表时间：
2014-11-01
影响因子：
3.2
通讯作者：
Fisher, Mark J.
中科院分区：
文献类型：
--
作者：
Liu, Shuo;Grigoryan, Mher Mahoney;Fisher, Mark J.
脑微出血是脑内血液制品沉积的显微镜下表现，可通过MRI观察到，与脑血管疾病有关。苏木精和伊红（H&E）和Perl的普鲁士蓝是流行的染色方法，用于定位病理学中的脑微出血。本文在小鼠脑微出血模型中比较了这两种染色技术。我们用脂多糖（LPS）诱导脑微血管病变。将C57 B6小鼠用LPS（5 mg/kg，i. p.）在第一次注射后48小时提取脑，并用H&E和普鲁士蓝对相邻冠状切片进行染色，以比较两种染色技术的有效性。H& E阳性染色增加与LPS治疗，并与脑表面上的肉眼可见的微血管造影;普鲁士蓝阳性染色，相比之下，没有显着增加与LPS治疗，并没有相关的H& E阳性染色或表面微血管造影。因此，H&E染色是该模型中在短时间跨度内由LPS诱导的急性出血事件的更可靠的指示剂。
Cerebral microbleeds are microscopic hemorrhages with deposits of blood products in the brain, which can be visualized with MRI and are implicated in cerebrovascular diseases. Hematoxylin and eosin (H&E) and Perl's Prussian blue are popular staining methods used to localize cerebral microbleeds in pathology. This paper compared these two staining techniques in a mouse model of cerebral microbleeds. We used lipopolysaccharide (LPS) to induce cerebral microhemorrhages. C57B6 mice were treated with LPS (5 mg/kg, i.p.) or vehicle at baseline and at 24 hr. The brains were extracted 48 hr after the first injection and adjacent coronal sections were stained with H&E and Prussian blue to compare the effectiveness of the two staining techniques. H&E-positive stains were increased with LPS treatment and were correlated with grossly visible microhemorrhages on the brain surface; Prussian blue-positive stains, by comparison, showed no significant increase with LPS treatment and did not correlate with either H&E-positive stains or surface microhemorrhages. H&E staining is thus a more reliable indicator of acute bleeding events induced by LPS in this model within a short time span.