Current advances in skin-on-a-chip models for drug testing.
Current advances in skin-on-a-chip models for drug testing.
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DOI：
10.21037/mps.2018.08.01
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发表时间：
2018-08
期刊：
影响因子：
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通讯作者：
Qiang Zhang;L. Sito;Mao Mao-Mao;Jiankang He;Yu Zhang;Xin Zhao
中科院分区：
文献类型：
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作者：
Qiang Zhang;L. Sito;Mao Mao-Mao;Jiankang He;Yu Zhang;Xin Zhao
与传统的 2D 细胞培养和动物模型相比，芯片皮肤模型在药物测试中非常受欢迎，因为它们可以以相对较低的成本复制器官特异性的 3D 结构组织和生理功能。为了设计生理相关的皮肤模型，人类皮肤结构已集成到微流体平台上，以构建可以模拟复杂体内情况的芯片皮肤系统。在这篇简短的评论中，我们首先简要介绍用于开发体外芯片皮肤模型的一些关键技术。然后，我们回顾了最先进的芯片皮肤模型在药物测试中的应用，重点是使用全层皮肤等效物（FTSE）模型、具有脉管系统、免疫细胞和毛囊等附加组件的皮肤模型以及多器官芯片模型。最后，我们讨论了复杂的、类体内皮肤芯片模型当前的一些挑战和未来的发展方向。
Skin-on-a-chip models are highly desirable in drug testing compared to conventional 2D cell culture and animal models as they can replicate organ-specific 3D structural organization and physiological functions at a relatively low cost. To engineer a physiologically relevant skin model, human skin structures have been integrated onto microfluidic platforms to construct skin-on-a-chip systems that can mimic the complex in vivo situation. In this mini-review, we first briefly introduce some critical technologies employed to develop in vitro skin-on-a-chip models. We then review the applications of the state-of-the-art skin-on-a-chip models in drug testing, with a focus on using models of full-thickness skin equivalents (FTSEs), skin models with additional components such as vasculature, immune cells and hair follicles as well as multi-organ-on-a-chip models. Finally, we discuss some current challenges and future directions of development of complex, and in vivo-like skin-on-a-chip models.