Glutamine Protects Intestinal Barrier Function of Colon Epithelial Cells from Ethanol by Modulating Hsp70 Expression
Glutamine Protects Intestinal Barrier Function of Colon Epithelial Cells from Ethanol by Modulating Hsp70 Expression
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DOI：
10.1159/000345930
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发表时间：
2013-01-01
期刊：
影响因子：
3.1
通讯作者：
Inouye, Sachiye
中科院分区：
文献类型：
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作者：
Akagi, Reiko;Ohno, Michiko;Inouye, Sachiye
在这项研究中，我们研究了谷氨酰胺对乙醇诱导的屏障功能障碍的保护作用，通过使用人上皮结直肠腺癌细胞（Caco-2）。我们的研究结果表明，添加谷氨酰胺的培养基显着提高了破坏的完整性所造成的乙醇，这是与热休克蛋白70（Hsp 70）的表达增加。乙醇暴露适度激活热休克因子1（HSF 1），其特征在于增加DNA结合活性和HSF 1的磷酸化状态。值得注意的是，谷氨酰胺处理增强了乙醇介导的Hsp 70表达和HSF 1活化。通过热应激预处理上调Hsp 70也促进了乙醇诱导的屏障功能障碍的恢复。总之，这些观察结果表明谷氨酰胺保护Caco-2细胞中的肠屏障功能，部分通过调节HSF 1介导的Hsp 70表达。版权所有（c）2013 S. Karger AG，巴塞尔
In this study, we investigated the protective effect of glutamine on barrier dysfunction induced by ethanol, by using human epithelial colorectal adenocarcinoma cells (Caco-2). Our results show that addition of glutamine to culture medium significantly improved the disruption of integrity caused by ethanol, which was associated with increased expression of heat shock protein 70 (Hsp70). Ethanol exposure moderately activates heat shock factor 1 (HSF1), which was characterized by increased DNA-binding activity and phosphorylation status of HSF1. Remarkably, glutamine treatment enhanced ethanol-mediated expression of Hsp70 and activation of HSF1. Up-regulation of Hsp70 by pretreatment with heat stress also promoted recovery from the ethanol-induced barrier dysfunction. Taken together, these observations indicate that glutamine protects the intestinal barrier function in Caco-2 cells, in part by modulating HSF1-mediated Hsp70 expression. Copyright (c) 2013 S. Karger AG, Basel