Multiple Campylobacter jejuni proteins affecting the peptidoglycan structure and the degree of helical cell curvature.
Multiple Campylobacter jejuni proteins affecting the peptidoglycan structure and the degree of helical cell curvature.
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DOI：
10.3389/fmicb.2023.1162806
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
空肠弯曲菌是一种革兰氏阴性螺旋菌。其螺旋形态，由肽聚糖（PG）层维持，在其在环境中的传播，定植和致病特性中起着关键作用。先前表征的PG水解酶Pgp 1和Pgp 2对于产生C.空肠螺旋形态，缺失突变体是杆状的，并且与野生型相比，其PG胞肽谱显示出改变。同源性搜索和生物信息学被用来确定其他的基因产物参与C。空肠形态发生：假定的细菌微丝蛋白1104和含有M23肽酶结构域的蛋白0166、1105和1228。相应基因的缺失导致不同的弯曲杆形态，其PG胞肽谱的变化。除了1104之外，突变体中的所有变化都是互补的。1104和1105的过表达也导致了形态学和胞肽谱的变化，表明这两种基因产物的剂量影响这些特征。相关的螺旋ε-变形杆菌幽门螺杆菌已表征了C. jejuni 1104、1105和1228蛋白的同源基因缺失。pylori对H. pylori PG胞肽谱和/或形态与C.空肠缺失突变体。因此，很明显，即使是具有相似形态和同源蛋白的相关生物也可以具有不同的PG生物合成途径，这突出了研究相关生物中PG生物合成的重要性。
Campylobacter jejuni is a Gram-negative helical bacterium. Its helical morphology, maintained by the peptidoglycan (PG) layer, plays a key role in its transmission in the environment, colonization, and pathogenic properties. The previously characterized PG hydrolases Pgp1 and Pgp2 are important for generating C. jejuni helical morphology, with deletion mutants being rod-shaped and showing alterations in their PG muropeptide profiles in comparison to the wild type. Homology searches and bioinformatics were used to identify additional gene products involved in C. jejuni morphogenesis: the putative bactofilin 1104 and the M23 peptidase domain-containing proteins 0166, 1105, and 1228. Deletions in the corresponding genes resulted in varying curved rod morphologies with changes in their PG muropeptide profiles. All changes in the mutants complemented except 1104. Overexpression of 1104 and 1105 also resulted in changes in the morphology and in the muropeptide profiles, suggesting that the dose of these two gene products influences these characteristics. The related helical ε-Proteobacterium Helicobacter pylori has characterized homologs of C. jejuni 1104, 1105, and 1228 proteins, yet deletion of the homologous genes in H. pylori had differing effects on H. pylori PG muropeptide profiles and/or morphology compared to the C. jejuni deletion mutants. It is therefore apparent that even related organisms with similar morphologies and homologous proteins can have diverse PG biosynthetic pathways, highlighting the importance of studying PG biosynthesis in related organisms.
影响因子：
6.7
作者：
Frirdich E;Biboy J;Adams C;Lee J;Ellermeier J;Gielda LD;Dirita VJ;Girardin SE;Vollmer W;Gaynor EC
通讯作者：
Gaynor EC