Oligodendrocyte precursors guide interneuron migration by unidirectional contact repulsion
Oligodendrocyte precursors guide interneuron migration by unidirectional contact repulsion
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DOI：
10.1126/science.abn6204
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发表时间：
2022-05-20
期刊：
影响因子：
56.9
通讯作者：
Nguyen, Laurent
中科院分区：
文献类型：
--
作者：
Lepiemme, Fanny;Stoufflet, Julie;Nguyen, Laurent
在前脑中，腹侧来源的少突胶质前体细胞(VOPC)与皮质中间神经元一起切向向皮质移动。在这里，我们在小鼠身上测试了这些种群在迁移过程中是否在胚胎发育过程中相互作用。通过将遗传模型的组织学分析与实时成像相结合，我们发现尽管它们都被趋化因子CXCL12所吸引，但vOPC和皮质中间神经元占据了富含这种趋化因子的相互排斥的前脑领土。此外，第一波vOPC耗竭选择性地扰乱了皮质中间神经元的迁移和分布。在细胞水平上，我们发现，通过促进单向接触排斥，第一波vOPC引导皮质中间神经元从它们都被吸引到的血管中迁移，从而允许中间神经元到达它们适当的皮质领土。
In the forebrain, ventrally derived oligodendrocyte precursor cells (vOPCs) travel tangentially toward the cortex together with cortical interneurons. Here, we tested in the mouse whether these populations interact during embryogenesis while migrating. By coupling histological analysis of genetic models with live imaging, we show that although they are both attracted by the chemokine Cxcl12, vOPCs and cortical interneurons occupy mutually exclusive forebrain territories enriched in this chemokine. Moreover, first-wave vOPC depletion selectively disrupts the migration and distribution of cortical interneurons. At the cellular level, we found that by promoting unidirectional contact repulsion, first-wave vOPCs steered the migration of cortical interneurons away from the blood vessels to which they were both attracted, thereby allowing interneurons to reach their proper cortical territories.