The effect of sperm miR-34c on human embryonic development kinetics and clinical outcomes
The effect of sperm miR-34c on human embryonic development kinetics and clinical outcomes
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精子 miR-34c 对人类胚胎发育动力学和临床结果的影响
DOI：
10.1016/j.lfs.2020.117895
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发表时间：
2020-09-01
期刊：
影响因子：
6.1
通讯作者：
Sun, Yingpu
中科院分区：
文献类型：
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作者：
Shi, Senlin;Shi, Qiongyao;Sun, Yingpu
目的：我们旨在研究精子 miR-34c 对早期人类胚胎发育动力学和体外受精 (IVF) 患者临床结局的影响。材料和方法：卵母细胞授精后，从 58 名接受 IVF 的患者中收集残留精子标本。通过 qRT-PCR 检测精子、卵母细胞、受精卵和胚胎/囊胚中的 miR-34c 表达水平，并使用延时技术观察胚胎发育动力学。为了证实 miR-34c 在调节早期胚胎发育中的作用，将 miR-34c siRNA 注射到从体外成熟卵母细胞获得的受精卵中。 ROC 曲线用于确定截止值。根据截止值对胚胎发育动力学和临床结果进行比较。主要发现：miR-34c 在 3PN 受精卵中表达水平最高，但在人卵母细胞中不表达。 miR-34c siRNA组胚胎发育动力学参数t2、t3、t4、t5显着延长，但卵裂率和优质胚胎率低于对照组。精子miR-34c水平与t5呈负相关，与囊胚形成率、优质囊胚率和妊娠率呈正相关。妊娠组的 miR-34c 水平和囊胚形成率较高（p < 0.05）。 Logistic回归分析显示精子miR-34c水平与妊娠显着相关（OR：5.056，95% CI：1.560-16.384；p = 0.007）。意义：精子miR-34c表达水平与胚胎发育动力学和临床结局相关。因此，miR-34c表达有利于胚胎发育，可作为IVF结果的指标。
Aims: We aimed to investigate the effect of sperm miR-34c on early human embryonic development kinetics and clinical outcomes of in vitro fertilization (IVF) patients.Materials and methods: After oocyte insemination, residual sperm specimens were collected from 58 patients undergoing IVF. miR-34c expression levels in sperm, oocytes, zygotes, and embryos/blastocysts were detected with qRT-PCR, and embryonic development kinetics were observed using time-lapse technology. To confirm the role of miR-34c in regulation of early embryonic development, miR-34c siRNA was injected into zygotes obtained from in vitro-matured oocytes. A ROC curve was used to determine the cutoff value. Comparisons of embryonic development kinetics and clinical outcomes were performed according to the cutoff value.Key findings: The miR-34c expression level was highest in 3PN zygotes, but was not expressed in human oocytes. In the miR-34c siRNA group, embryonic development kinetic parameters t2, t3, t4, and t5 were significantly prolonged, but the cleavage rate and high-quality embryo rate were lower than in the control group. The levels of sperm miR-34c were negatively correlated with t5 and positively correlated with rates of blastocyst formation, high-quality blastocysts, and pregnancy. The miR-34c levels and the blastocyst formation rate were higher in the pregnancy group (p < 0.05). Logistic regression analysis showed that sperm miR-34c level was significantly correlated with pregnancy (OR: 5.056, 95% CI: 1.560-16.384; p = 0.007).Significance: The sperm miR-34c expression level is associated with embryonic development kinetics and clinical outcomes. Thus, miR-34c expression is beneficial to embryonic development and may be used as an indicator of IVF outcomes.