Enantioselectivity of toxicological responses induced by maternal exposure of cis-bifenthrin enantiomers in zebrafish (Dario rerio) larvae
Enantioselectivity of toxicological responses induced by maternal exposure of cis-bifenthrin enantiomers in zebrafish (Dario rerio) larvae
复制标题
斑马鱼（Dario rerio）幼虫母体暴露顺式联苯菊酯对映体引起的毒理学反应的对映选择性
DOI：
10.1016/j.jhazmat.2019.03.049
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发表时间：
2019
影响因子：
13.6
通讯作者：
Wang Qiangwei
中科院分区：
文献类型：
--
作者：
Xiang D;an;Qiao Kun;Song Zhuoying;Shen Shuyuan;Wang Mengcen;Wang Qiangwei
手性污染物的母体转移和发育毒性与对映体选择性的关系很少被研究。本文研究了典型手性农药cis-BF在成年斑马鱼体内的残留和毒性反应，结果表明，cis-BF对映体在斑马鱼体内的母体转移潜力相当，在斑马鱼体内的残留量为0、20、100和500 ng/L。GC/MSD分析表明，亲本1 S-cis-BF暴露可破坏子代胚胎脂肪酸组分。在转录表达方面，差异表达基因主要集中在GO类基因，包括与脂类生物合成/代谢相关的基因。脂肪酸的扰动表明，顺式BF对胚胎发育有潜在的负面影响。此外，还观察到对映体选择性生长抑制和发育神经毒性。1 S暴露后，仔虫神经元发育相关基因的mRNA表达、神经递质水平和幼虫运动能力均发生显著变化。我们的研究结果表明，thecis-BF诱导斑马鱼幼虫的生长抑制和神经毒性，这可能是介导的胚胎发育干扰有关的脂肪酸组成的破坏。此外，在暴露成人的后代中，对1 S-cis-BF的毒理学反应大于对1 R-对映体的毒理学反应。
The maternal transfer and developmental toxicity of chiral contaminants with respect to enantioselectivity have rarely been investigated. Here, the residues and toxicological responses ofcis-BF, a typical chiral pesticide, were studied in the progeny of adult zebrafish exposed tocis-BF (0, 20, 100, and 500 ng/L) for 60 days.Cis-BF enantiomers exhibited the equal maternal transfer potentials. GC/MSD analysis showed that parental 1S-cis-BF exposure could disrupt the components of fatty acids in offspring embryos. In transcriptional expression, the whole differentially expressed genes were significantly enriched in GO categories, including the processes related to lipid biosynthesis/metabolism. The perturbations of fatty acids suggested thatcis-BF has potential negative impacts on embryos’ development. Furthermore, enantioselective growth inhibition and developmental neurotoxicity in larvae were also observed. The mRNA expressions of neuronal development genes were significantly changed in 1S-exposed offspring, so were the levels of the neurotransmitters and larval locomotion. Our results show that thecis-BF induced the growth inhibition and neurotoxicity in zebrafish larvae, which may be mediated by the development interference in embryos related to the disrupted fatty acid composition. Furthermore, the toxicological response to 1S-cis-BF was greater than that to the 1R-enantiomer in the offspring of exposed adults.