Reversible stimulation of the Na+/K+ pump by monensin in cultures of vascular smooth muscle.
Reversible stimulation of the Na+/K+ pump by monensin in cultures of vascular smooth muscle.
复制标题
血管平滑肌培养物中莫能菌素对 Na /K 泵的可逆刺激。
DOI：
10.1016/0024-3205(82)90177-1
复制
发表时间：
1982
期刊：
影响因子：
6.1
通讯作者：
Smith,JB
中科院分区：
文献类型：
--
作者：
Brock,TA;Smith,JB
两种离子载体莫能菌素和盐霉素可增加大鼠主动脉平滑肌细胞培养物中总细胞 Na+ 和哇巴因敏感的 86Rb+ 的摄取。莫能菌素用于产生细胞 Na+ 的分级增加，以评估完整细胞中 Na+/K+ 泵的 Na+ 依赖性。内部 Na+ 和哇巴因敏感的 86Rb+ 摄取之间的关系是双曲线的 (K1Na = 3 mM)。在没有外部Na+的情况下，莫能菌素不刺激86Rb+的吸收。通过将培养物暴露于不含 K+ 的培养基 3 小时，用 Na+ 负载细胞，最大程度地增加细胞 Na+ 和哇巴因敏感的 86Rb+ 摄取，达到与莫能菌素相同的程度。去除离子载体后，莫能菌素处理的细胞中的总细胞 Na+ 和泵活性恢复到初始值。然后，莫能菌素能够将总细胞 Na+ 和哇巴因敏感的 86Rb+ 摄取增加到与离子载体初始治疗相同的程度。
Two ionophores, monensin and salinomycin, increased total cell Na+and ouabain-sensitive86Rb+uptake in cultures of smooth muscle cells from rat aorta. Monensin was used to produced graded increases in cell Na+in order to assess the Na+dependence of the Na+/K+pump in the intact cell. The relationship between internal Na+and ouabain-sensitive86Rb+uptake was hyperbolic (K1Na = 3 mM). Monensin did not stimulate86Rb+uptake in the absence of external Na+. Loading the cells with Na+by exposing cultures to a K+-free medium for 3 hr maximally increased cell Na+and ouabain-sensitive86Rb+uptake to the same extent as monensin. Total cell Na+and pump activity in monensin-treated cells returned to the initial values after removing the ionophore. Monensin was then able to increase total cell Na+and ouabain-sensitive86Rb+uptake to the same extent as the initial treatment with the ionophore.