ACTIVATION OF HIV GENE-EXPRESSION DURING MONOCYTE DIFFERENTIATION BY INDUCTION OF NF-KAPPA-B
ACTIVATION OF HIV GENE-EXPRESSION DURING MONOCYTE DIFFERENTIATION BY INDUCTION OF NF-KAPPA-B
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DOI：
10.1038/339070a0
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发表时间：
1989-05-04
期刊：
影响因子：
64.8
通讯作者：
NABEL, GJ
中科院分区：
文献类型：
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作者：
GRIFFIN, GE;LEUNG, K;NABEL, GJ
艾滋病的潜伏期受到激活人类免疫缺陷病毒(HIV)在不同细胞类型中复制的因素的影响。虽然单核细胞可能为病毒在活体1-8中的产生提供了一个储存库，但它们对HIV转录的调节还没有被确定。我们现在报道，单核细胞系中HIV基因的表达受核因子-kB的调节，核因子-kB是已知在激活的T细胞中刺激艾滋病毒增强子的同一转录因子9；然而，单核细胞中对核因子-kB和艾滋病毒的控制与在T细胞中观察到的不同。在体外诱导分化的U937细胞中，在原单核细胞向单核细胞的转变过程中出现了核因子-kB结合活性，并且在成熟的单核细胞和巨噬细胞中呈结构性存在。在慢性感染的前单核细胞Ul中，分化与HIV-1复制和NF-kB结合活性有关。这些发现表明，核因子-kB结合活性在单核细胞系中是发育调节的，它为这些细胞中的HIV激活提供了一个信号。
THE latent period of AIDS is influenced by factors which activate human immunodeficiency virus (HIV) replication in different cell types. Although monocytic cells may provide a reservoir for virus productionin vivo1–8, their regulation of HIV transcription has not been defined. We now report that HIV gene expression in the monocyte lineage is regulated by NF-kB, the same transcription factor known to stimulate the HIV enhancer in activated T cells9; however, control of NF-kB and HIV in monocytes differs from that observed in T cells. NF-kB-binding activity appears during the transition from promonocyte to monocyte in U937 cells induced to differentiatein vitroand is present constitutively in mature monocytes and macrophages. In a chronically infected pro-monocytic cell, Ul, differentiation is associated with HIV-1 replication as well as NF-kB binding activity. These findings suggest that NF-kB binding activity is developmentally regulated in the monocyte lineage, and that it provides one signal for HIV activation in these cells.