Production and characterization of monoclonal antibodies to cholera toxin
Production and characterization of monoclonal antibodies to cholera toxin
复制标题
霍乱毒素单克隆抗体的制备和表征
DOI：
10.1128/iai.37.1.70-76.1982
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发表时间：
1982
影响因子：
3.1
通讯作者：
R. Goldsby
中科院分区：
文献类型：
--
作者：
E. Remmers;R. Colwell;R. Goldsby
制备了抗霍乱毒素的单克隆抗体，以获得高度特异性的抗霍乱毒素血清。用霍乱毒素免疫小鼠，取其脾细胞与骨髓瘤细胞融合，获得15株分泌霍乱毒素决定簇特异性单克隆抗体的杂交瘤细胞株。将细胞系稳定化，检查特异性抗体产生，并通过冷冻培养的细胞和在小鼠皮下生长的肿瘤细胞使其永生化。所有细胞系在解冻后继续分泌抗体。通过测定轻链和重链组分的类别以及通过测定霍乱毒素亚单位的特异性来表征杂交瘤细胞系产生的抗体。所有抗体均含有k轻链，4个含有mu重链，其余11个含有γ 1重链。其中10个单克隆抗体对霍乱毒素的B亚单位具有特异性，3个对A亚单位具有特异性。剩下的两个似乎与两个亚单位反应。
Monoclonal antibodies against cholera toxin were produced to obtain highly specific antisera to cholera toxin. Fifteen hybridoma cell lines producing monoclonal antibodies specific for the determinants of cholera toxin were derived from the fusion of mouse myeloma cells and spleen cells from mice immunized with cholera toxin. The cell lines were stabilized, examined for specific antibody production, and immortalized by freezing cultured cells and tumor cells which had been grown subcutaneously in mice. All cell lines continued antibody secretion upon thawing. The antibodies produced by the hybridoma cell lines were characterized by determination of the class of light- and heavy-chain components and by determination of specificity for the cholera toxin subunit. All of the antibodies contained the k light chain, 4 contained the mu heavy chain, and the remaining 11 contained the gamma 1 heavy chain. Ten of the monoclonal antibodies are specific for the B subunit of cholera toxin, and three are specific for the A subunit. The remaining two appear to react with both subunits.