Visualization of Spatial Distribution of Spermatogenesis in Mouse Testes Using Creatine Chemical Exchange Saturation Transfer Imaging
Visualization of Spatial Distribution of Spermatogenesis in Mouse Testes Using Creatine Chemical Exchange Saturation Transfer Imaging
复制标题
肌酸化学交换饱和传递成像技术研究小鼠睾丸精子发生的空间分布
DOI：
10.1002/jmri.27734
复制
发表时间：
2021-05-30
影响因子：
4.4
通讯作者：
Sakata, Yasushi
中科院分区：
文献类型：
--
作者：
Takahashi, Yusuke;Kioka, Hidetaka;Sakata, Yasushi
背景在确定男性不育的治疗策略时，目的探讨肌酸化学交换饱和转移(CrCEST)成像在评价精子发生及其空间分布中的作用。研究类型展望：动物模型C57BL/6对照小鼠(n=5)和全睾丸X射线照射(n=11)或睾丸下部局部X射线照射(n=3)所致男性不育模型小鼠(n=3)。场强/序列A 11.7-T垂直孔磁共振成像(MRI)/分段快速低角度激发对CEST的评估计算对照组小鼠和X射线照射模型小鼠每个睾丸中CrCEST效应的磁化传递比(MTRCr*)。照射后15、20、30天。MTRcr*与Johnsen评分(精子发生的组织学评分)之间进行相关分析。在局部X射线照射模型中，计算区域MTRcr*和Johnsen评分进行相关分析，统计检验采用非配对t检验，单因素方差分析采用Tukey‘s HSD检验，相关分析采用Pearson’s相关分析。结果在照射模型中，CRCEST显像显示MTRCr线性下降(对照组，8.7+/-0.6；10天，7.9+/-0.8；15天，6.5+/-0.6；20天，5.4+/-1.0；30天，4.4+/-0.8)。MTRcr*与Johnsen‘s评分呈显著线性相关(r=0.79)。在局部照射模型中，CrCEST成像显示非屏蔽区MTRcr*在非屏蔽区显著降低(屏蔽区为6.9+/-0.7；非屏蔽区为4.9+/-1.0)，并且区域MTRcr*与Johnsen评分呈显著线性相关(r=0.78)。CrCEST成像有助于评估精子发生及其空间分布。证据级别2技术疗效阶段2。
Background When determining treatment strategies for male infertility, it is important to evaluate spermatogenesis and its spatial distribution in the testes.Purpose To investigate the usefulness of creatine chemical exchange saturation transfer (CrCEST) imaging for evaluating spermatogenesis and its spatial distribution.Study Type Prospective.Animal Model C57BL/6 control mice (n = 5) and model mice of male infertility induced by whole testis X-ray irradiation (n = 11) or localized X-ray irradiation to lower regions of testes (n = 3).Field Strength/Sequence A 11.7-T vertical-bore magnetic resonance imaging (MRI)/segmented fast low-angle shot acquisition for CEST.Assessment The magnetization transfer ratio for the CrCEST effect (MTRCr*) was calculated in each testis of the control mice and X-ray irradiation model mice at 10, 15, 20, and 30 days after irradiation. Correlation analysis was performed between MTRCr* and Johnsen's score, a histological score for spermatogenesis. In the localized X-ray irradiation model, regional MTRCr* and Johnsen's score were calculated for correlation analysis.Statistical Tests Unpaired t-test, one-way analysis of variance with Tukey's HSD test and Pearson's correlation analysis. A P value < 0.05 was considered statistically significant.Results In the irradiation model, CrCEST imaging revealed a significant linear decrease of MTRCr* after irradiation (control, 8.7 +/- 0.6; 10 days, 7.9 +/- 0.8; 15 days, 6.5 +/- 0.6; 20 days, 5.4 +/- 1.0; 30 days, 4.4 +/- 0.8). A significant linear correlation was found between MTRCr* and Johnsen's score (Pearson's correlation coefficient (r) = 0.79). In the localized irradiation model, CrCEST imaging visualized a significant regional decrease of MTRCr* in the unshielded region (shielded, 6.9 +/- 0.7; unshielded, 4.9 +/- 1.0), and a significant linear correlation was found between regional MTRCr* and Johnsen's score (r = 0.78).Data Conclusion Testicular CrCEST effects correlated well with spermatogenesis. CrCEST imaging was useful for evaluating spermatogenesis and its spatial distribution.Evidence Level 2Technical Efficacy Stage 2.