Correlation between the expression of apoptosis-related bcl-2 and p53 oncoproteins and the carcinogenesis and progression of breast carcinomas.
Correlation between the expression of apoptosis-related bcl-2 and p53 oncoproteins and the carcinogenesis and progression of breast carcinomas.
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凋亡相关bcl-2和p53癌蛋白的表达与乳腺癌发生、发展的相关性。
DOI：
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发表时间：
1997
影响因子：
11.5
通讯作者：
A. Tsuchiya
中科院分区：
文献类型：
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作者：
Guo;I. Kimijima;Rikiya Abe;M. Kanno;N. Katagata;K. Hara;Takanori Watanabe;A. Tsuchiya
据报道，原癌基因 bcl-2 在乳腺组织中表达，该基因通过抑制细胞凋亡来调节细胞死亡。野生型 p53 已被证明可诱导细胞凋亡，而 bcl-2 表达可抑制细胞凋亡。然而，bcl-2和p53表达在乳腺癌发生中的作用尚未阐明。本研究的目的是利用免疫组织化学方法评估正常乳腺上皮细胞以及乳腺癌组织的导管内和浸润性癌病变中bcl-2和p53的表达，并阐明它们在乳腺癌发生发展中的作用。还通过定量图像分析评估了 p53 的核积累。分别在 82 个正常导管上皮细胞中的 79 个 (96%)、63 个导管内癌中的 50 个 (79%) 以及 137 个浸润性癌中的 62 个 (45%) 中发现了 bcl-2 的表达。正常上皮细胞中的 bcl-2 表达高于导管内和侵袭性癌细胞（P < 0.0001）。此外，导管内病变中的bcl-2阳性率显着高于浸润性癌性病变（P < 0.05）。在正常乳腺上皮细胞中未观察到 p53 核积聚。 63 个导管内癌性病变中的 15 个 (23.8%) 和 137 个浸润性癌性病变中的 41 个 (30%) p53 表达呈阳性。浸润性癌中 bcl-2 和 p53 的表达呈负相关。我们证明bcl-2表达存在于大多数正常导管上皮细胞中，并在乳腺癌的发展过程中逐渐减少，即从正常上皮到导管内癌，以及从导管内癌到浸润性癌，而p53表达可能在乳腺癌发展早期出现并在进展过程中增加。
The proto-oncogene bcl-2, which is implicated in the regulation of cell death by inhibiting apoptosis, is reported to be expressed in breast tissues. The wild-type p53 has been shown to induce apoptosis, which can be inhibited by bcl-2 expression. However, the role of bcl-2 and p53 expression in breast carcinogenesis has not been clarified. The purpose of this study was to evaluate bcl-2 and p53 expression in normal breast epithelia cells, as well as in intraductal and invasive cancerous lesions of breast cancer tissue using an immunohistochemical method and to clarify their role in the development of breast cancer. The nuclear accumulation of p53 was also evaluated by quantitative image analysis. Expression of bcl-2 was found in 79 of 82 (96%) normal ductal epithelial cells, in 50 of 63 (79%) intraductal carcinomas, and in 62 of 137 (45%) invasive carcinomas, respectively. Higher bcl-2 expression was observed in normal epithelial cells than in intraductal and invasive cancerous cells (P < 0.0001). Furthermore, bcl-2 positivity in intraductal lesions was significantly higher than in invasive cancerous lesions (P < 0.05). No p53 nuclear accumulation was observed in normal breast epithelial cells. Fifteen of 63 (23.8%) intraductal cancerous lesions and 41 of 137 (30%) invasive cancerous lesions were positive for p53 expression. An inverse relationship was shown between bcl-2 and p53 expression in invasive carcinomas. We demonstrated that bcl-2 expression exists in most of normal ductal epithelial cells and gradually decreases during the development of breast cancer, i.e. , from a normal epithelium to intraductal carcinoma, and from intraductal to invasive carcinoma, and that p53 expression may occur early in breast cancer development and increases during progression.