Embryonic expression and cloning of the murine GATA-3 gene.
Embryonic expression and cloning of the murine GATA-3 gene.
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发表时间：
1994-09
期刊：
影响因子：
4.6
通讯作者：
Kathleen M. George;M. Leonard;Matthew E. Roth;Kenneth Lieuw;Dimitris Kioussis;F. Grosveld;J. D. Engel
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文献类型：
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作者：
Kathleen M. George;M. Leonard;Matthew E. Roth;Kenneth Lieuw;Dimitris Kioussis;F. Grosveld;J. D. Engel
我们描述了小鼠（m）加塔-3基因的胚胎表达模式以及克隆和初步的转录调控分析。原位杂交显示，mGATA-3 mRNA的积累在早期发育过程中受到时间和空间上的调节：尽管在胚胎发生前10天的胎盘中发现了最丰富的mGATA-3表达，但在妊娠后期，mGATA-3表达仅限于胚胎中枢神经系统（中脑、间脑、脑桥和内耳）内的特定细胞。加塔-3在外周神经系统中也显示出受限的表达模式，包括颅神经节和交感神经节中的终末分化细胞。除了在神经系统中的这种独特模式之外，mGATA-3还在胚胎肾和胸腺原基中表达，并且进一步的分析表明其在整个T淋巴细胞分化中表达。为了开始研究这种复杂的基因表达模式是如何引起的，开始了mGATA-3基因的克隆和转录调控分析。在将m加塔-3定向报告基因转染到天然表达加塔-3的细胞（T淋巴细胞和神经母细胞瘤细胞）中后，适当的组织限制性调节似乎需要至少两种调节元件（一种阳性和一种阴性）。此外，该基因座的相同区域赋予转基因小鼠发育适当的表达，但仅在天然表达该基因的组织的子集中。
We describe the embryonic expression pattern as well as the cloning and initial transcriptional regulatory analysis of the murine (m) GATA-3 gene. In situ hybridization shows that mGATA-3 mRNA accumulation is temporally and spatially regulated during early development: although found most abundantly in the placenta prior to 10 days of embryogenesis, mGATA-3 expression becomes restricted to specific cells within the embryonic central nervous system (in the mesencephalon, diencephalon, pons and inner ear) later in gestation. GATA-3 also shows a restricted expression pattern in the peripheral nervous system, including terminally differentiating cells in the cranial and sympathetic ganglia. In addition to this distinct pattern in the nervous system, mGATA-3 is also expressed in the embryonic kidney and the thymic rudiment, and further analysis showed that it is expressed throughout T lymphocyte differentiation. To begin to investigate how this complex gene expression pattern is elicited, cloning and transcriptional regulatory analyses of the mGATA-3 gene were initiated. At least two regulatory elements (one positive and one negative) appear to be required for appropriate tissue-restricted regulation after transfection of mGATA-3-directed reporter genes into cells that naturally express GATA-3 (T lymphocytes and neuroblastoma cells). Furthermore, this same region of the locus confers developmentally appropriate expression in transgenic mice, but only in a subset of the tissues that naturally express the gene.