Subcellular localisation of the middle and large T‐antigens of polyoma virus.
Subcellular localisation of the middle and large T‐antigens of polyoma virus.
复制标题
多瘤病毒中、大T抗原的亚细胞定位。
作者：
S. Dilworth;H. Hansson;C. Darnfors;G. Bjursell;C. Streuli;B. Griffin
使用已充分表征的单克隆抗体，通过间接免疫荧光和免疫电子显微镜研究了两种多瘤病毒早期蛋白（大 T 抗原和中 T 抗原）在裂解感染小鼠细胞和转化大鼠细胞中的分布。通过这些技术，病毒大 T 抗原几乎只在细胞核中发现，有时与核孔相关，但从未在核仁中发现。在裂解感染但未转化的细胞中，在细胞核内的离散区域（“热点”）中检测到荧光，并且在少数裂解感染细胞中，观察到细胞质免疫反应物质。病毒中 T 抗原与大多数细胞质膜相关，并且在大多数细胞中主要存在于内质网中。在质膜中仅观察到一小部分染色，而在核质中未观察到染色。数据表明，多瘤病毒主要转化活性的作用位点不一定位于质膜。与病毒抗原相关的功能根据其在细胞内的亚细胞分布进行讨论。
The distribution of two of the polyoma virus early proteins (the large and middle T‐antigens) in lytically infected mouse cells and transformed rat cells has been investigated by indirect immunofluorescence and immuno‐electron microscopy using well‐characterised monoclonal antibodies. By these techniques, the viral large T‐antigen was found almost exclusively in the nucleus, sometimes in association with nuclear pores, but never in the nucleolus. In lytically infected, but not transformed cells, fluorescence was detected in discrete areas (‘hot spots’) within the nucleus and, in a minor population of lytically infected cells, cytoplasmic immunoreactive material was observed. The viral middle T‐antigen was found in association with most cytoplasmic membranes and in the majority of cells mainly in the endoplasmic reticulum. Only a fraction of the staining was observed in the plasma membrane and no staining in the nucleoplasm was observed. The data suggest that the site of action of the major transforming activity of polyoma virus need not be at the plasma membrane. Functions associated with the viral antigens are discussed in terms of their subcellular distributions within cells.