Lessons from optical tweezers: quantifying organelle interactions, dynamics and modelling subcellular events.
Lessons from optical tweezers: quantifying organelle interactions, dynamics and modelling subcellular events.
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DOI：
10.1016/j.pbi.2018.07.010
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发表时间：
2018-12
影响因子：
9.5
通讯作者：
I. Sparkes
中科院分区：
文献类型：
--
作者：
I. Sparkes
光镊（Optical tweezers，OT）是一种用于捕获植物细胞器的物理工具。它可以用来确定植物细胞器的定位和相互作用的功能作用。OT可以用来量化驱动细胞器运动的力分量。OT彻底改变了我们对植物细胞器动力学的理解。光镊使用户能够物理捕获细胞器并在植物细胞内横向移动它们。最近的进展突出了功能相关的细胞器对，如ER-高尔基体和过氧化物酶体叶绿体之间的物理相互作用，并显示了细胞器的定位如何影响植物生长。这些过程的量化提供了洞察力的组件，最终驱动细胞器的运动和定位在植物细胞。因此，光镊的应用彻底改变了我们对植物细胞器动力学的理解。
HighlightsOptical tweezers (OT) allow users to physically trap plant organelles in vivo.Functional role of organelle positioning and interaction can be determined using OT.OT can be used to quantify force components driving organelle movement.OT have revolutionised our understanding of plant organelle dynamics.Optical tweezers enable users to physically trap organelles and move them laterally within the plant cell. Recent advances have highlighted physical interactions between functionally related organelle pairs, such as ER–Golgi and peroxisome–chloroplast, and have shown how organelle positioning affects plant growth. Quantification of these processes has provided insight into the force components which ultimately drive organelle movement and positioning in plant cells. Application of optical tweezers has therefore revolutionised our understanding of plant organelle dynamics.