Probing single secretory vesicles with capillary electrophoresis
Probing single secretory vesicles with capillary electrophoresis
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DOI：
10.1126/science.279.5354.1190
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发表时间：
1998-02-20
期刊：
影响因子：
56.9
通讯作者：
Lundqvist, JA
中科院分区：
文献类型：
--
作者：
Chiu, DT;Lillard, SJ;Lundqvist, JA
通过结合光学捕获、毛细管电泳分离和激光诱导荧光检测，对从腹足类软体动物海兔的心房腺中获得的分泌囊泡进行单独的化学分析。利用光学捕获技术，将体积为阿托升（10（-18）升）的单个囊泡引入分离毛细管的锥形入口，囊泡注射后即被裂解，分离前用萘-2,3-二甲醛对其成分进行荧光标记，所得电泳图显示单个囊泡的含量存在明显变化。
Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individually with a combination of optical trapping, capillary electrophoresis separation, and a laser-induced fluorescence detection. With the use of optical trapping, a single Vesicle that had attoliters (10(-18) liters) of volume was introduced into the tapered inlet of a separation capillary, Once the vesicle was injected, it was lysed, and its components were fluorescently labeled with naphthalene-2,3-dicarboxaldehyde before separation, The resultant electropherograms indicated distinct variations in the contents of single vesicles.