Chromosomal localization of ev-1, a frequently occurring endogenous retrovirus locus in white Leghorn chickens, by in situ hybridization
Chromosomal localization of ev-1, a frequently occurring endogenous retrovirus locus in white Leghorn chickens, by in situ hybridization
复制标题
通过原位杂交对白来亨鸡中常见的内源逆转录病毒位点 ev-1 进行染色体定位
作者：
Allan;'. Tereba;S. Astrin
对来自不同地理来源的13个白色来航鸡群共508只鸡的12个内源性逆转录病毒位点的分布进行了检测。只有一个基因座，命名为ev-1，被观察到几乎普遍存在（508只鸡中的506只），因此在这个品种的鸡中主要是纯合子。相比之下，其他11个病毒位点的观察频率要低得多，3至30%，偶尔仅限于个别鸡群。为了进一步研究ev-1病毒基因座的特征，并为确定这12个内源性逆转录病毒基因座产生的机制提供基础，我们采用了一种新的间接原位杂交技术来定位ev-1的染色体位置。通过使用特异性探针的组合，ev-1的位置被定位到1号染色体长臂上的一个小区域。这项研究代表了第一批通过原位杂交将编码一组已知基因的非重复DNA的定义片段定位到脊椎动物染色体上的特定区域的实例。
The distribution of 12 endogenous retrovirus loci from 13 White Leghorn flocks of diverse geographical origin was examined, using a total of 508 birds. Only one locus, designated ev-1, was observed to be almost universally present (506 of 508 birds) and thus predominantly homozygous in this breed of chicken. By contrast, 11 other viral loci were observed at much lower frequencies, 3 to 30%, and were occasionally restricted to individual flocks. To further characterize the commonly occurring ev-1 viral locus and to provide a foundation for determining the mechanism by which these 12 endogenous retrovirus loci were generated, a new technique of indirect in situ hybridization was used to localize the chromosomal position of ev-1. By using a combination of specific probes, the position of ev-1 was localized to a small region on the long arm of chromosome 1. This research represents oen of the first instances in which a defined segment of nonreiterated DNA coding for a known set of genes has been localized to a specific region on a vertebrate chromosome by in situ hybridization.