Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs
Two opposing roles for Bmp signalling in the development of electrosensory lateral line organs
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DOI：
10.1101/2024.03.07.583945
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发表时间：
2024-05
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影响因子：
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通讯作者：
Alexander S. Campbell;Martin Minařík;Roman Franěk;Michaela Vazačová;Miloš Havelka;David Gela;M. Pš
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文献类型：
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作者：
Alexander S. Campbell;Martin Minařík;Roman Franěk;Michaela Vazačová;Miloš Havelka;David Gela;M. Pš
侧线系统使所有鱼类和水生阶段的两栖动物能够通过神经乳突中的机械感觉毛细胞检测局部水运动，并且许多物种能够通过壶腹器官中的电感受器（修饰的毛细胞）检测弱电场。神经丘和壶腹器官都是由侧线基板发育而来。然而，作为壶腹器官形成的基础的分子机制相对于神经瘤而言研究不足，因为斑马鱼（硬骨鱼）和爪蟾（青蛙）的祖先谱系独立地失去了电感受。我们通过差异RNA-seq在一种电感受性鳍鱼，密西西比白鲟（Polyodon spathula; Modrell et al.，2017，eLife 6：e24197）。在实验上听话的相对，鲟鱼（鲟），我们发现，BMP 5和其他四个BMP通路基因表达在发展侧线，BMP信号是活跃的。此外，CRISPR/Cas9介导的在G 0注射的Sterlet胚胎中靶向Bmp 5的诱变导致较少的壶腹器官。相反，当BMP信号被抑制DMH 1治疗前不久壶腹器官原基的形成，多余的壶腹器官的发展。这些数据表明Bmp 5促进壶腹器官发育，而Bmp通过另一种配体发出信号可以防止其过度产生。两者合计，这证明了两个相反的作用，在壶腹器官形成的BMP信号。
The lateral line system enables all fishes and aquatic-stage amphibians to detect local water movement via mechanosensory hair cells in neuromasts, and many species to detect weak electric fields via electroreceptors (modified hair cells) in ampullary organs. Both neuromasts and ampullary organs develop from lateral line placodes. However, the molecular mechanisms underpinning ampullary organ formation are understudied relative to neuromasts, as the ancestral lineages of zebrafish (teleosts) and Xenopus (frogs) independently lost electroreception. We identified Bmp5 as a promising candidate via differential RNA-seq in an electroreceptive ray-finned fish, the Mississippi paddlefish (Polyodon spathula; Modrell et al., 2017, eLife 6: e24197). In an experimentally tractable relative, the sterlet sturgeon (Acipenser ruthenus), we found that Bmp5 and four other Bmp pathway genes are expressed in the developing lateral line, and that Bmp signalling is active. Furthermore, CRISPR/Cas9-mediated mutagenesis targeting Bmp5 in G0-injected sterlet embryos resulted in fewer ampullary organs. Conversely, when Bmp signalling was inhibited by DMH1 treatment shortly before the formation of ampullary organ primordia, supernumerary ampullary organs developed. These data suggest that Bmp5 promotes ampullary organ development, whereas Bmp signalling via another ligand(s) prevents their overproduction. Taken together, this demonstrates two opposing roles for Bmp signalling during ampullary organ formation.