Imaging Mass Spectrometry Reveals Unique Lipid Distribution in Primary Varicose Veins
Imaging Mass Spectrometry Reveals Unique Lipid Distribution in Primary Varicose Veins
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DOI：
10.1016/j.ejvs.2010.08.001
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发表时间：
2010-11-01
影响因子：
5.7
通讯作者：
Setou, M.
中科院分区：
文献类型：
--
作者：
Tanaka, H.;Zaima, N.;Setou, M.
背景：静脉曲张（VVs）的脂质代谢尚不清楚。为了阐明VV的发病机制，我们采用了成像质谱（IMS）的新技术。材料和方法：我们从10例大隐静脉剥离患者的10个肢体中获得W组织。作为对照静脉样本，我们采集了6例行腹股沟下搭桥逆行隐静脉移植术的外周动脉闭塞性疾病患者的6个肢体的节段静脉组织。为了确定脂质分子在W组织中的定位，我们进行了基质辅助激光解吸/电离IMS （MALDI-IMS）。我们还进行了MS/MS分析，以确定每个分子的结构。结果：我们直接从对照静脉组织和VV组织中获得质谱，发现脂质分子在VV组织中有独特的定位。我们将溶血磷脂酰胆碱（LPC）（1-酰基16:0）、磷脂酰胆碱（PC）（1-酰基36:4）和鞘磷脂（SM） （d18:1/16:0）定位于VV瓣膜部位。结论：MALDI-IMS显示了正常静脉和静脉内各种脂质分子的分布。VV组织中LPC（1-酰基16:0）、PC（1-酰基36:4）和SM （d18:1/16:0）的积累提示脂质代谢异常相关的炎症可能参与了VV的发生。(C) 2010年欧洲血管外科学会。Elsevier Ltd.出版。版权所有。
Background: The lipid metabolism of varicose veins (VVs) remains unknown. To elucidate the pathogenesis of VV, we utilized the novel technique of imaging mass spectrometry (IMS).Materials and methods: We obtained W tissues from 10 limbs of 10 W patients who underwent great saphenous vein stripping. As control vein samples, we harvested segmental vein tissues from 6 limbs of 6 patients with peripheral artery occlusive disease who underwent infra-inguinal bypass with reversed saphenous vein grafting. To identify the localisation of lipid molecules in the W tissues, we performed matrix-assisted laser desorption/ionization IMS (MALDI-IMS). We also performed MS/MS analyses to identify the structure of each molecule.Results: We obtained mass spectra directly from control vein tissues and VV tissues and found a unique localisation of lipid molecules in the VV tissues. We localised lysophosphatidylcholine (LPC) (1-acyl 16:0), phosphatidylcholine (PC) (1-acyl 36:4) and sphingomyelin (SM) (d18:1/16:0) at the site of the VV valve.Conclusion: MALDI-IMS revealed the distribution of various lipid molecules in normal veins and VVs both. Accumulation of LPC (1-acyl 16:0), PC (1-acyl 36:4) and SM (d18:1/16:0) in the VV tissues suggested that inflammation associated with abnormal lipid metabolism may contribute to the development of VV. (C) 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.