RHIZOBIUM-MELILOTI NTRA (RPON) GENE IS REQUIRED FOR DIVERSE METABOLIC FUNCTIONS
RHIZOBIUM-MELILOTI NTRA (RPON) GENE IS REQUIRED FOR DIVERSE METABOLIC FUNCTIONS
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DOI：
10.1128/jb.169.6.2424-2431.1987
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发表时间：
1987-06-01
影响因子：
3.2
通讯作者：
AUSUBEL, FM
中科院分区：
文献类型：
--
作者：
RONSON, CW;NIXON, BT;AUSUBEL, FM
本文报道了苜蓿根瘤菌中一个类似ntrA的基因（glnF rpoN）的鉴定和克隆。苜蓿属植物ntrA产物（NtrA）是C4-二羧酸盐运输以及硝酸盐同化和共生固氮所必需的。DNA序列分析表明，R.苜蓿NtrA与肺炎克雷伯氏菌NtrA的同源性为38%。亚克隆和互补序列分析表明，该基因为一个新的表达载体。苜蓿ntrA启动子位于起始密码子的125个碱基对内，并且可以组成型表达。
We report the identification and cloning of an ntrA-like (glnF rpoN) gene of Rhizobium meliloti and show that the R. meliloti ntrA product (NtrA) is required for C4-dicarboxylate transport as well as for nitrate assimilation and symbiotic nitrogen fixation. DNA sequence analysis showed that R. meliloti NtrA is 38% homologous with Klebsiella pneumoniae NtrA. Subcloning and complementation analysis suggested that the R. meliloti ntrA promoter lies within 125 base pairs of the initiation codon and may be constitutively expressed.