Synthesis and characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis
Synthesis and characterization of a small, membrane-permeant, caspase-activatable far-red fluorescent peptide for imaging apoptosis
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DOI：
10.1021/jm050008p
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发表时间：
2005-08-25
影响因子：
7.3
通讯作者：
Piwnica-Worms, D
中科院分区：
文献类型：
--
作者：
Bullok, K;Piwnica-Worms, D
为了用小的膜渗透探针在体内成像细胞凋亡，合成TcapQ（647），其包含基于Tat肽的渗透肽序列、效应物半胱天冬酶识别序列DEVD和侧翼光学可活化对，所述侧翼光学可活化对包含远红猝灭剂QSY 21和荧光团Alexa Fluor 647。在基线条件下，观察到高猝灭效率，导致低背景荧光。在暴露于执行器半胱天冬酶后，TcapQ（647）被特异性切割，从而从猝灭剂释放荧光团并使细胞凋亡成像成为可能。
To image apoptosis in vivo with a small, membrane-permeant probe, TcapQ(647) was synthesized comprising a Tat-peptide-based permeation peptide sequence, an effector caspase recognition sequence, DEVD, and a flanking optically activatable pair comprising a far-red quencher, QSY 21, and a fluorophore, Alexa Fluor 647. Under baseline conditions, high quenching efficiencies were observed resulting in low background fluorescence. Upon exposure to executioner caspases, TcapQ(647) was specifically cleaved, thereby releasing the fluorophore from the quencher and enabling imaging of apoptosis.