Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2)-infected pneumonia (COVID-19)
Comparison of throat swabs and sputum specimens for viral nucleic acid detection in 52 cases of novel coronavirus (SARS-Cov-2)-infected pneumonia (COVID-19)
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DOI：
10.1515/cclm-2020-0187
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发表时间：
2020-07-01
影响因子：
6.8
通讯作者：
Shen, Changxin
中科院分区：
文献类型：
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作者：
Lin, Chenyao;Xiang, Jie;Shen, Changxin
目的：2019年12月，中国武汉发生新型冠状病毒（SARS-CoV-2）感染的肺炎（COVID-19）。基于实验室的诊断测试利用咽喉样本的实时逆转录酶聚合酶链反应（RT-PCR）。方法：收集54例患者咽拭子和痰标本，提取RNA进行2019-nCoV检测，分析咽拭子和痰标本对2019-nCoV的诊断价值结果：痰标本和咽拭子标本中2019-nCoV的阳性率分别为76.9%和44.2%。结论：痰标本中2019-nCoV的检出率明显高于咽拭子标本，痰标本中2019-nCoV的检出率明显高于咽拭子标本。我们认为，在产生痰液的患者中，痰液将有利于2019-nCoV的检测。本研究结果有助于标本的选择和提高诊断的准确性。
Objectives: In December 2019, a novel coronavirus (SARS-CoV-2)-infected pneumonia (COVID-19) occurred in Wuhan, China. Laboratory-based diagnostic tests utilized real-time reverse transcriptase polymerase chain reaction (RT-PCR) on throat samples. This study evaluated the diagnostic value to analyzing throat and sputum samples in order to improve accuracy and detection efficiency.Methods: Paired specimens of throat swabs and sputum were obtained from 54 cases, and RNA was extracted and tested for 2019-nCoV (equated with SARS-CoV-2) by the RT-PCR assay.Results: The positive rates of 2019-nCoV from sputum specimens and throat swabs were 76.9% and 44.2%, respectively. Sputum specimens showed a significantly higher positive rate than throat swabs in detecting viral nucleic acid using the RT-PCR assay (p = 0.001).Conclusions: The detection rates of 2019-nCoV from sputum specimens were significantly higher than those from throat swabs. We suggest that sputum would benefit for the detection of 2019-nCoV in patients who produce sputum. The results can facilitate the selection of specimens and increase the accuracy of diagnosis.