Mass Spectrometry for Paper-Based Immunoassays: Toward On-Demand Diagnosis
Mass Spectrometry for Paper-Based Immunoassays: Toward On-Demand Diagnosis
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DOI：
10.1021/jacs.6b02232
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发表时间：
2016-05-25
影响因子：
15
通讯作者：
Badu-Tawiah, Abraham K.
中科院分区：
文献类型：
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作者：
Chen, Suming;Wan, Qiongqiong;Badu-Tawiah, Abraham K.
目前的分析方法，无论是即时检测还是集中检测，都无法满足最近对患者友好的检测和增加结果可靠性的需求。在这里，我们描述了一种基于纸基质谱免疫分析平台的两点分离按需诊断策略，该平台采用稳定且可裂解的离子探针作为质量报告子;这些探针使灵敏、可中断、可储存和可重复的按需检测成为可能。此外，还开发了一种新的触纸喷雾方法，用于芯片上、灵敏且具有成本效益的分析物检测。通过（i）检测恶性疟原虫富组氨酸蛋白2抗原和（ii）多重和同时检测癌抗原125和癌胚抗原，成功地证明了这一概念。
Current analytical methods, either point-of-care or centralized detection, are not able to meet recent demands of patient-friendly testing and increased reliability of results. Here, we describe a two-point separation on-demand diagnostic strategy based on a paper-based mass spectrometry immunoassay platform that adopts stable and cleavable ionic probes as mass reporter; these probes make possible sensitive, interruptible, storable, and restorable on-demand detection. In addition, a new touch paper spray method was developed for on-chip, sensitive, and cost-effective analyte detection. This concept is successfully demonstrated via (i) the detection of Plasmodium falciparum histidine-rich protein 2 antigen and (ii) multiplexed and simultaneous detection of cancer antigen 125 and carcinoembryonic antigen.